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UTDallas/25 October 2010
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===October 25, 2010 === | ===October 25, 2010 === | ||
| - | * | + | *Performed titration of Pu+GFP, Pr+XylR in 0M, 10uM, 500uM, 10mM, and 100mM xylene, benzene, and toluene |
*Incubated LacI+RFP, PyeaR+GFP overnight for testing tomorrow | *Incubated LacI+RFP, PyeaR+GFP overnight for testing tomorrow | ||
| + | ;Alkane Sensor | ||
| + | *Set up another digestion of the midipreped plasmis of part E0240 with XbaI. | ||
| + | *65C 10mins to heat kill XbaI | ||
| + | *Nucleotide removal kit after that to cleanup the DNA | ||
| + | *Tried blunt End ligation. So did blunt ending with Klenow fragment of the Vector(E0240) and the insert (PAlk Promoter) | ||
| + | *Nucleotide removal kit after that to cleanup the DNA of both vector ans the insert | ||
| + | *Setup ligation at room temperature for 1 hour. | ||
| + | *Transformed into Dh5Alpha cells and plated them in 50ug/ml agar plate. | ||
Latest revision as of 20:48, 27 October 2010
Notebook
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October 25, 2010
- Performed titration of Pu+GFP, Pr+XylR in 0M, 10uM, 500uM, 10mM, and 100mM xylene, benzene, and toluene
- Incubated LacI+RFP, PyeaR+GFP overnight for testing tomorrow
- Alkane Sensor
- Set up another digestion of the midipreped plasmis of part E0240 with XbaI.
- 65C 10mins to heat kill XbaI
- Nucleotide removal kit after that to cleanup the DNA
- Tried blunt End ligation. So did blunt ending with Klenow fragment of the Vector(E0240) and the insert (PAlk Promoter)
- Nucleotide removal kit after that to cleanup the DNA of both vector ans the insert
- Setup ligation at room temperature for 1 hour.
- Transformed into Dh5Alpha cells and plated them in 50ug/ml agar plate.
