Team:Hong Kong-CUHK/Project results

We had successfully constructed vector containing RCI recombination system and encoded message. In first trial, we encoded a short message:

"we must learn to live together as brothers or perish together as tools"

in a single vector, together with two inverted repeats. We designed primers which targets encoded message either in normal orientation or reverse-complementary orientation. Both sets of primers could be used to generate PCR products, indicating that encoded message exists in both recombinated and normal forms. Sequencing results confirmed the correctness of the PCR product.