UTDallas/19 October 2010
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(New page: *Test digestion and then gel electrophoresis for this weekend's ligations: results were good. All the parts were at the expected length *Digested the random part (BBa_J04450) with XbaI and...) |
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+ | {{Template:UTDallasTop}} | ||
+ | {{Template:UTDallasMenu}} | ||
+ | <span style="color:#EDE8E8"> | ||
+ | <html> | ||
+ | <style type="text/css"> | ||
+ | table, tr, td {background:transparent; border:0px;} | ||
+ | table table table {background-color:COLOR;} */to change background color from white to transparent | ||
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+ | table.calendar { margin: 0; padding: 0px; background-color:#660099} | ||
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+ | table.month .dow td { color:#660099; text-align:center; font-size:90%; } | ||
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+ | #bodyContent table.month a { background:#none; padding:0 } | ||
+ | .day-active { color:#FF8000} | ||
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+ | </style> | ||
+ | </html> | ||
+ | |||
+ | ==Notebook== | ||
+ | |||
+ | {| align="center" | ||
+ | |-align="top" | ||
+ | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=06}} | ||
+ | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=07}} | ||
+ | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=08}} | ||
+ | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=09}} | ||
+ | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=10}} | ||
+ | |align="center" width="150pt"|{{#calendar: title=UTDallas |year=2010 | month=11}} | ||
+ | |} | ||
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+ | |||
+ | |||
+ | ===October 19, 2010 === | ||
+ | ;Nitrate Sensor | ||
*Test digestion and then gel electrophoresis for this weekend's ligations: results were good. All the parts were at the expected length | *Test digestion and then gel electrophoresis for this weekend's ligations: results were good. All the parts were at the expected length | ||
*Digested the random part (BBa_J04450) with XbaI and PstI to get just the backbone, pSB1C3, then ran gel electrophoresis (took only the top band ~2050bp) and ran gel purification: results were good. | *Digested the random part (BBa_J04450) with XbaI and PstI to get just the backbone, pSB1C3, then ran gel electrophoresis (took only the top band ~2050bp) and ran gel purification: results were good. | ||
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*Ligated the pSB1C3 to PyeaR+GFP and PyeaR+RFP | *Ligated the pSB1C3 to PyeaR+GFP and PyeaR+RFP | ||
*Incubated GFP overnight for miniprepping and digestion tomorrow to ligate to the alkane sensor | *Incubated GFP overnight for miniprepping and digestion tomorrow to ligate to the alkane sensor | ||
+ | ;Alkane Sensor | ||
+ | * Midi prep on the overnight grown cells having part E0240. |
Latest revision as of 20:12, 27 October 2010
Notebook
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October 19, 2010
- Nitrate Sensor
- Test digestion and then gel electrophoresis for this weekend's ligations: results were good. All the parts were at the expected length
- Digested the random part (BBa_J04450) with XbaI and PstI to get just the backbone, pSB1C3, then ran gel electrophoresis (took only the top band ~2050bp) and ran gel purification: results were good.
- Both parts were ran on the same gel. The test digestion is the left three bands and the pSB1C3 is the right bands.
- Ligated the pSB1C3 to PyeaR+GFP and PyeaR+RFP
- Incubated GFP overnight for miniprepping and digestion tomorrow to ligate to the alkane sensor
- Alkane Sensor
- Midi prep on the overnight grown cells having part E0240.