Team:METU Turkey/Results Discussion/CooA Expression and Purification

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<br> D. Ferric Citrate effect
<br> D. Ferric Citrate effect
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<br> E. Soluble versus insoluble fraction
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<br> 2-Purification optimization
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<br> A. Ion exchange chromatography with Sepharose Q (QS)
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Revision as of 18:58, 27 October 2010

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3-CooA Expression and Purification



1-Overexpression optimization
A. Expression vector comparison (pKK versus pTriEx)



B. Expression host comparison (JM109 versus BL21)



C. IPTG induction studies


D. Ferric Citrate effect


E. Soluble versus insoluble fraction


2-Purification optimization


A. Ion exchange chromatography with Sepharose Q (QS)




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