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Team:Tsinghua/Notebook/8 October 2010
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(New page: == Module I, Group 2c == Digest the purified products and plasmid PSB1C13 with EcoR I and Pst I. ---- Double digestion system eGFP 5ul EcoR I 1ul Pst I ...) |
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| + | == Module I, Group 1A == | ||
| + | Extract plasmid ptv. Transform into competent with pAH69 through electrotransformation. Spread to 0.03% tetracyclin plates. | ||
| + | |||
== Module I, Group 2c == | == Module I, Group 2c == | ||
Digest the purified products and plasmid PSB1C13 with EcoR I and Pst I. | Digest the purified products and plasmid PSB1C13 with EcoR I and Pst I. | ||
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FD Buffer 2ul | FD Buffer 2ul | ||
H2O 12ul | H2O 12ul | ||
| - | + | ||
| + | ---- | ||
| + | |||
Kan 4ul | Kan 4ul | ||
EcoR I 1ul | EcoR I 1ul | ||
Latest revision as of 15:01, 27 October 2010
Module I, Group 1A
Extract plasmid ptv. Transform into competent with pAH69 through electrotransformation. Spread to 0.03% tetracyclin plates.
Module I, Group 2c
Digest the purified products and plasmid PSB1C13 with EcoR I and Pst I.
Double digestion system
eGFP 5ul EcoR I 1ul Pst I 1ul FD Buffer 2ul H2O 11ul
mCherry 4ul EcoR I 1ul Pst I 1ul FD Buffer 2ul H2O 12ul
Kan 4ul EcoR I 1ul Pst I 1ul FD Buffer 2ul H2O 12ul
Incubate at 37 centidegree overnight.
