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Team:Newcastle/Meetings/21 October 2010
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| - | ==Formal Meeting - 21st October== | + | ==Formal Meeting - 21st October 2010== |
*Apologies - Phil, Younus, Deena (late) | *Apologies - Phil, Younus, Deena (late) | ||
| + | *[[Team:Newcastle/Agendas/21_October_2010|Agenda]] | ||
====''yneA'' Characteristaion:==== | ====''yneA'' Characteristaion:==== | ||
| - | *Δ''amyE'':Pspac(hy)::''yneA'' | + | *Δ''amyE'':Pspac(hy)-oid::''yneA'' |
*''B. subtilis'' spread of IPTG may not be homogenous through the population. | *''B. subtilis'' spread of IPTG may not be homogenous through the population. | ||
| Line 26: | Line 27: | ||
*Younus AP: send script for yneA to Jen, Matt, Neil | *Younus AP: send script for yneA to Jen, Matt, Neil | ||
| - | *Plot: Max cell length against ITPG + Error bars | + | *Plot: Max cell length against ITPG + Error bars |
====Arabinose==== | ====Arabinose==== | ||
Latest revision as of 10:44, 27 October 2010
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Contents |
Formal Meeting - 21st October 2010
- Apologies - Phil, Younus, Deena (late)
- Agenda
yneA Characteristaion:
- ΔamyE:Pspac(hy)-oid::yneA
- B. subtilis spread of IPTG may not be homogenous through the population.
- E. coli has transporter deltions: check E. coli wiki
- take out pMap65 results
- Median, Mode add to graph
- pMutin4 proportion of cells
- 20% responding- looks like
- Biomass of cells - length * amount (counts)) against IPTG
- Lysis acts to counter the overexpression of yneA ... so we have fewer cells
- Younus AP: send script for yneA to Jen, Matt, Neil
- Plot: Max cell length against ITPG + Error bars
Arabinose
- We think we have it cloned infront of GFP
- B. subtilis transformation tommorrow
- Fluorescence
- Characterisation of previous part!!!
Concrete
- Damaged building - earthquake
Flux balance
- difference between optimal and normal
Lab work
- DNA submitted - tracking number sent
- 1C3 SR1 has not arrived, we would have had 2 bricks submitted!
Safety page
- Deena and Harsh
- Risk assessment - Bacillus
- BioCOSH
- Safety Guy talk mention it
Presentation
- Phil - DON'T BE AGGRESSIVE!!!!
- Beginning:
- say what the project is about
- mention Bacillus subtilis
- Why bacillus?
- bioconcrete- show its current
- Non-pathogenic not non-virulent
- BioBricks:
- Previous work on CaCO3
- Don't use the word PROVES
- Alan - Flux first, speak slowly
- Gold:
- Jannetta write RFC
- number on judging form
- webservice!!!
- Separate slid for work flows
- SEM:
- Images are ours
- Summary table
- redo with BRONZE, SILVER, GOLD section?!
Action points:
- Check presentation and poster submission. Poster next Thursday due in?
- Phil and Rachel to add characteristation data to the wiki
Other
- Insurance form
- Taxi
   
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