Team:Newcastle/Meetings/21 October 2010

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(New page: Apologies - Phil, Younus, Deena late Action point: Phil and Rachel add characteristation data to the wiki ==''yneA'' Characteristaion:== *Δ''amyE'':Pspac(hy)::''yneA'' *''Bacillus sub...)
 
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Apologies - Phil, Younus, Deena late
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{{Team:Newcastle/mainbanner}}
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Action point: Phil and Rachel add characteristation data to the wiki
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==Formal Meeting - 21st October 2010==
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==''yneA'' Characteristaion:==
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*Apologies - Phil, Younus, Deena (late)
 +
*[[Team:Newcastle/Agendas/21_October_2010|Agenda]]
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*Δ''amyE'':Pspac(hy)::''yneA''
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====''yneA'' Characteristaion:====
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*''Bacillus subtilis'' spread of IPTG may not be homogenous through the population.
+
*Δ''amyE'':Pspac(hy)-oid::''yneA''
-
*''E.coli'' has transporter deltions: check ''E.coli'' wiki
+
-
take out pMap65 results
+
*''B. subtilis'' spread of IPTG may not be homogenous through the population.
 +
*''E. coli'' has transporter deltions: check ''E. coli'' wiki
-
Median, Mode add to graph
+
*take out pMap65 results
-
pMutin4 proportion of cells
+
*Median, Mode add to graph
-
20% responding- looks like
+
*pMutin4 proportion of cells
-
Biomass of cells - length * amount(counts)) against IPTG
+
*20% responding- looks like
-
Lysis acts to counter the overexpression of yneA ... so we have fewer cells  
+
*Biomass of cells - length * amount (counts)) against IPTG
-
'''Younus!!!''' AP: send script for yneA to Jen, Matt, Neil
+
*Lysis acts to counter the overexpression of yneA ... so we have fewer cells
-
Plott: Max cell length against ITPG + Error bars
+
*Younus AP: send script for yneA to Jen, Matt, Neil
-
==Arabinose==
+
*Plot: Max cell length against ITPG + Error bars
-
We think we have it cloned infront of GFP
+
====Arabinose====
-
''Bacillus subtilis'' transformation tommorrow
+
-
Fluorescence
+
-
'''Characterisation of previous part!!!'''
+
-
==Concrete==
+
* We think we have it cloned infront of GFP
 +
*''B. subtilis'' transformation tommorrow
 +
*Fluorescence
 +
*Characterisation of previous part!!!
-
Damaged building- earthquake
+
====Concrete====
-
==Flux balance==
+
* Damaged building - earthquake
-
difference between optimal and normal
+
====Flux balance====
-
==Lab work==
+
*difference between optimal and normal
-
DNA submitted... tracking number sent
+
====Lab work====
-
1C3 ''SR1'' has not arrived, we would have had 2 bricks submitted!
+
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==Safety page==
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*DNA submitted - tracking number sent
 +
*1C3 ''SR1'' '''has not arrived''', we would have had 2 bricks submitted!
-
Deena and Harsh
+
====Safety page====
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Risk assessment- ''Bacillus''
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*Deena and Harsh
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BioCOSH
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*Risk assessment - ''Bacillus''
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Safety Guy talk mention it
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*BioCOSH
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==Presentation==
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*Safety Guy talk mention it
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'''Phil''' DON'T BE AGGRESSIVE!!!!
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====Presentation====
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Begining:  
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*Phil - DON'T BE AGGRESSIVE!!!!
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*say what the project is about  
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*Beginning:  
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*mention ''Bacillus subtilis''
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**say what the project is about  
-
*Why bacillus  
+
**mention ''Bacillus subtilis''
-
*bioconcrete- show its current
+
**Why bacillus?
 +
**bioconcrete- show its current
 +
**Non-pathogenic not non-virulent
 +
*BioBricks:
 +
**Previous work on CaCO<sub>3</sub>
 +
**Don't use the word '''PROVES'''
 +
**Alan - Flux first, speak slowly
 +
*Gold:
 +
**Jannetta write RFC
 +
**number on judging form
 +
**webservice!!!
 +
**Separate slid for work flows
 +
*SEM:
 +
**Images are ours
 +
*Summary table
 +
**redo with BRONZE, SILVER, GOLD section?!
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Non-pathogenic not non-virulent
+
====Action points:====
-
Previous work on CaCO3
+
*Check presentation and poster submission. Poster next Thursday due in? 
-
Don't use the word '''PROVES'''
+
*Phil and Rachel to add characteristation data to the wiki
-
Separate slid for work flows
+
-
Alan- Flux first, speak slowly
+
====Other====
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Summary table- redo
+
*Insurance form
 +
*Taxi
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BRONZE, SILVER, GOLD!!!!!!!
+
{{Team:Newcastle/footer}}
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+
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===GOLD=
+
-
 
+
-
Jannetta write RFC-
+
-
*number on judging form
+
-
*webservice!!!
+
-
 
+
-
===SEM===
+
-
 
+
-
Images are ours
+
-
 
+
-
==Other==
+
-
 
+
-
Insurance form
+
-
Taxi
+
-
 
+
-
'''Younus''' Poster!!! next thursday due in? 
+
-
 
+
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Action point check presnetation and poster submission!!!
+

Latest revision as of 10:44, 27 October 2010

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Contents

Formal Meeting - 21st October 2010

  • Apologies - Phil, Younus, Deena (late)
  • Agenda

yneA Characteristaion:

  • ΔamyE:Pspac(hy)-oid::yneA
  • B. subtilis spread of IPTG may not be homogenous through the population.
  • E. coli has transporter deltions: check E. coli wiki
  • take out pMap65 results
  • Median, Mode add to graph
  • pMutin4 proportion of cells
  • 20% responding- looks like
  • Biomass of cells - length * amount (counts)) against IPTG
  • Lysis acts to counter the overexpression of yneA ... so we have fewer cells
  • Younus AP: send script for yneA to Jen, Matt, Neil
  • Plot: Max cell length against ITPG + Error bars

Arabinose

  • We think we have it cloned infront of GFP
  • B. subtilis transformation tommorrow
  • Fluorescence
  • Characterisation of previous part!!!

Concrete

  • Damaged building - earthquake

Flux balance

  • difference between optimal and normal

Lab work

  • DNA submitted - tracking number sent
  • 1C3 SR1 has not arrived, we would have had 2 bricks submitted!

Safety page

  • Deena and Harsh
  • Risk assessment - Bacillus
  • BioCOSH
  • Safety Guy talk mention it

Presentation

  • Phil - DON'T BE AGGRESSIVE!!!!
  • Beginning:
    • say what the project is about
    • mention Bacillus subtilis
    • Why bacillus?
    • bioconcrete- show its current
    • Non-pathogenic not non-virulent
  • BioBricks:
    • Previous work on CaCO3
    • Don't use the word PROVES
    • Alan - Flux first, speak slowly
  • Gold:
    • Jannetta write RFC
    • number on judging form
    • webservice!!!
    • Separate slid for work flows
  • SEM:
    • Images are ours
  • Summary table
    • redo with BRONZE, SILVER, GOLD section?!

Action points:

  • Check presentation and poster submission. Poster next Thursday due in?
  • Phil and Rachel to add characteristation data to the wiki

Other

  • Insurance form
  • Taxi
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