Team:Tec-Monterrey/Instructors

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<ul>
<ul>
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li>
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Under">Undergraduates</a></li>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors</a></li>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Instructors">Instructors/Advisors</a></li>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Attributions">Attributions</a></li>
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li>
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Headquarters">Headquarters</a></li>
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<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">&nbsp;&nbsp;Project</a></p>
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<a href="#">&nbsp;&nbsp;Project</a></p>
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<ul>
<ul>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Project">Project Overview</a></li>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Introduction">Introduction</a></li>
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                         <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Biosensor">The Biosensor</a></li>
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                         <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Geneticframe">Genetic Frame</a></li>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Biosensor">Applications</a></li>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Parts">Parts</a></li>
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<li><a href="https://2010.igem.org/Team:Tec-Monterrey/FutureResearch">Future Research</a></li>
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<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Documentation">&nbsp;&nbsp;Documentation</a></p>
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<a href="#">&nbsp;&nbsp;Notebook</a></p>
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                         <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Notebook">Notebook</a></li>
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                         <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Journal">Journal</a></li>
                         <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li>
                         <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Protocols">Protocols</a></li>
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        <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Timeline">Timeline</a></li>
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        <li><a href="https://2010.igem.org/Team:Tec-Monterrey/SafetyEthics">Safety & Ethics</a></li>
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                         <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Humanpractices">Human Practices</a></li>
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<p><br/><a href="https://2010.igem.org/Team:Tec-Monterrey/Bonus">&nbsp;&nbsp;Bonus</a></p>
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<ul>
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                        <li><a href="https://2010.igem.org/Team:Tec-Monterrey/News">News</a></li>
 
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li>
<li><a href="https://2010.igem.org/Team:Tec-Monterrey/Gallery">Gallery</a></li>
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                        <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Freetime">Free Time</a></li>
 
                         <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li>
                         <li><a href="https://2010.igem.org/Team:Tec-Monterrey/Mascot">Wiki Wiki</a></li>
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<h2>  Sponsors:</h2>
 
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<a href="http://www.uniparts.com.mx/" target="blank"><img src="https://static.igem.org/mediawiki/2010/d/d9/Uniparts.jpg" width="200px" height="83px" border="0" ></a>
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<a href="http://www.promega.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/2/20/Promega.gif" width="170px" height="110px" border="0" ></a> 
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<a href="http://www.corning.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/3/32/Corning.jpg" width="170px" height="29px" border="0" ></a>
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<a href="http://www.sartorius.com/" target="blank"><img src=" https://static.igem.org/mediawiki/2010/7/70/Sartoriussmall.jpg" border="0" ></a>
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<a href="http://www.millipore.com/"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a>
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<a href="http://www.millipore.com/" target="blank"><img src="https://static.igem.org/mediawiki/2010/6/6b/Millipore.jpg" width="170px" height="50px" border="0" ></a>
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<a href="http://www.sukarne.com.mx/"><img src="https://static.igem.org/mediawiki/2010/6/6a/Sukarne.gif" width="120px" height="120px" border="0" ></a>
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<h2>Follow us on:</h2>
 
<a href="http://tinyurl.com/2wvxmdu"><img src="https://static.igem.org/mediawiki/2010/a/a5/Twitter-ogo.png" width="175px" height="55px" border="0" ></a>
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<h1>Project Summary</h1>
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<h2>Dr. Mario Moisés Álvarez</h2>
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<img src="https://static.igem.org/mediawiki/2010/9/9b/Drmario.jpg" alt="Dr. Mario Álvarez" />
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Mario Moisés Álvarez is the former director of the FEMSA Biotechnology Center at the Tecnológico de Monterrey in México. He is the coordinator of the Biotechnology Graduate Program in this same institution, and one of the youngest scientists in México ranked as a Level 2 Researcher by the Mexican System of Researchers (SNI). Dr. Álvarez has a Ph.D. from Rutgers University in Chemical and Biochemical Engineering, a Masters in Science in Chemical Engineering from Rutgers University, and a Masters in Science in Process Engineering and a Bachelor Degree in Biochemistry from the Tecnológico de Monterrey. He did a Postdoctoral stay at Bristol Myers Squibb, at the Pharmaceutical Research Institute, where he collaborated in different projects related to the design and scale-up of Pharmaceutical Processes, particularly reactive and crystallization processes.
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</p>
<p class="blah">
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      Bacterial reporters or whole-cell bacterial sensors have always been an area of application for genetic manipulation and synthetic biology. As a matter of fact, constructing a bioreporter bacteria that has the ability to detect toxic chemicals is considered one of the first accomplishments in the discipline of synthetic biology <a href="#van">(van der Meer and Belkin, 2010)</a>. There are several advantages to using a bacterial bioreporter instead of a traditional physical or chemical sensors, for example, bacteria can offer the same specificity and sensitivity that traditional sensors offer, but they are much more portable and grow in inexpensive media. Furthermore, bacterial reporters also offer advantages over using other types of biosensors like enzymes and antibodies because they are living organisms and they are capable of analyzing samples through a process that involves many enzymes <a href="#yagi">(Yagi, 2007)</a>
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Dr. Álvarez's main area of scientific expertise is laminar mixing (mixing operations at low speed or in high viscosity conditions). Currently, his group is working in the application of mixing knowledge on the design of bioreactors. This includes the design of novel high performance bioreactor systems for animal cell culture, the diagnose of mixing performance in bioreactors through reactive methods, and the study of non-ideal mixing conditions in bioreactive systems.
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      The first bacterial reporters appeared 20 years ago <a href="#van">(van der Meer and Belkin, 2010)</a> although these early tests didn’t use genetically modified microorganisms. Further research and development in the areas of genetic engineering and synthetic biology have resulted in many more applications such as detection of contaminants <a href="#willardson">(Willardson, et. Al., 1998)</a> and sugar and amino acid availability in soils <a href="#jaeger">(Jaeger, et. Al., 1999)</a>. Even though the reporters have gotten more sophisticated and sensitive, we realized that there isn’t much mention of a single bacterial bioreporter capable of detecting different concentrations of a substance and reacting differently depending on the concentration.  
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      We thought the use of synthetic biology as well as the BioBrick standard could help create a “genetic circuit” <a href="#van">(van der Meer and Belkin, 2010)</a> capable of detecting different concentrations of a substance and reacting in a different manner depending on the amount detected. In our initial research we realized that the iGEM British Columbia 2009 team decided to do something similar, so we used parts of their project as a base and integrated parts of other previous iGEM projects in order to propose a new genetic construction capable of detecting different amounts of a certain substance. We call these new types of sensors, “intelligent biosensors”, because they have the ability to react in different ways depending on their surroundings.
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      With our project we hope to continue with the previous efforts of other iGEM teams, and at the same time propose a new type of genetic circuit for achieving these functions. In the process we plan to develop BioBricks for two new families of phage activators as well as different BioBrick constructons that can make our system easy to adapt, so that the creation of these “intelligent biosensors” becomes just a matter of choosing the substance of interest and choosing the different reporters.
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<h2>M. Sc. Sergio Ángel García Echauri</h2>
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<img src="https://static.igem.org/mediawiki/2010/a/a6/Sergio.jpg" alt="M. Sc. Sergio García" />
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Sergio Ángel García Echauri is a researcher in the FEMSA Biotechnology Center at the Tecnológico de Monterrey in México, where he is currently working in his Ph.D. His early formation was in the Autonomous University of Baja California, where he obtained his title as a Biologist. He then carried out a thesis on the Isolation and Identification of Psychrophillic Organisms from the Antarctica and Expression of a Superoxide Dismutase from <i>Deschampsia antarctica</i> in <i>Escherichia coli</i>.
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During the influenza outbreak in 2009, Sergio was part of the team that developed a novel technique for its detection and the creation of a recombinant vaccine against the A/H1N1. He was then invited to the Novartis Fourth Annual Global BioCamp, an event for the biotechnology leaders from the best universities in 27 countries, held at Cambridge, Massachusets in October 2009.
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<h2>Dr. Manuel I. Zertuche Guerra</h2>
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Dr. Manuel Zertuche Guerra has a Masters Degree in Science with a specialization in Production Systems and a Ph.D. in Plant Physiology from Texas A&M.  In 1992, he was named the Head of the International Center for Agribusinesses and then became the Director of the Center of Strategic Studies in the Tecnológico de Monterrey. He developed strategic planning projects in Venezuela, Ecuador, Argentina, Bolivia, Nicaragua, and El Salvador. He works as a consultant and has participated in several conferences and seminars in the United States and Canada. He is currently the Head of the Biotechnology and Food Division in the Tecnológico de Monterrey and leads several research projects in collaboration with important Mexican corporations, such as FEMSA, CEMEX, LALA, Tyson, and Landsteiner Scientific.
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José Manuel Aguilar majored in Biology with a specialty in Molecular Biology at the Baja California Autonomous University. In 2007, he got a M.S. Degree in Biotechnology at the Tecnológico de Monterrey, where he was awarded an Honorary Mention for Excellence and an Outstanding Thesis Mention. He has two scientific publications and two more under revision, as well as five national patent requests and one Patent Cooperation Treaty. As of now, he works at the Tecnológico de Monterrey as a professor and is on his way to finish his Ph.D.
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<p class="blah"><a name="jaeger">Jaeger, C. H., et. Al. (June 1999)</a> Mapping of Sugar and Amino Acid Availability in Soil around Roots with Bacterial Sensors of Sucrose and Tryptophan. Applied and Environmental Microbiology, Vol. 65, No. 6, p. 2685 - 2690
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<a name="van">Van der Meer, J. R. and Belkin, S. (July 2010)</a> Where microbiology meets microengineering: design and applications of reporter bacteria. Nature Reviews Microbiology, Vol. 8, p. 511 - 522
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Johari Salgado is a biologist graduated from the Baja California Autonomous University. In 2007, he received an M.S. Degree in Biotechnology at the Tecnológico de Monterrey. He participated in the development of an Influenza vaccine during the  A/H1N1 2009 outbreak and currently leads several projects in collaboration with Landsteiner Scientific, in addition to teaching the Genetic Engineering Laboratory in the Tecnológico de Monterrey.  
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Felipe López Pacheco has a technical degree in Biotechnology and is currently majoring in Biotechnology Engineering. As part of a joint project between the Landsteiner Pharmaceutical Group and ITESM, he is in charge of performing ELISAS to find antibodies.
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<a name="willardson">Willardson, B. M., et. Al. (March 1998)</a> Development and Testing of a Bacterial Biosensor for Toluene-Based Environmental Contaminants. Applied and Environmental Microbiology, Vol. 64, No. 3, p. 1006- 1012
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<a name="yagi">Yagi, K. (2007)</a> Applications of whole-cell bacterial sensors in biotechnology and environmental science. Applied Microbiology and Biotechnology, Vol. 73, p. 1251 - 1258
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Tec de Monterrey






























Dr. Mario Moisés Álvarez

Dr. Mario Álvarez Mario Moisés Álvarez is the former director of the FEMSA Biotechnology Center at the Tecnológico de Monterrey in México. He is the coordinator of the Biotechnology Graduate Program in this same institution, and one of the youngest scientists in México ranked as a Level 2 Researcher by the Mexican System of Researchers (SNI). Dr. Álvarez has a Ph.D. from Rutgers University in Chemical and Biochemical Engineering, a Masters in Science in Chemical Engineering from Rutgers University, and a Masters in Science in Process Engineering and a Bachelor Degree in Biochemistry from the Tecnológico de Monterrey. He did a Postdoctoral stay at Bristol Myers Squibb, at the Pharmaceutical Research Institute, where he collaborated in different projects related to the design and scale-up of Pharmaceutical Processes, particularly reactive and crystallization processes.

Dr. Álvarez's main area of scientific expertise is laminar mixing (mixing operations at low speed or in high viscosity conditions). Currently, his group is working in the application of mixing knowledge on the design of bioreactors. This includes the design of novel high performance bioreactor systems for animal cell culture, the diagnose of mixing performance in bioreactors through reactive methods, and the study of non-ideal mixing conditions in bioreactive systems.

M. Sc. Sergio Ángel García Echauri

M. Sc. Sergio García Sergio Ángel García Echauri is a researcher in the FEMSA Biotechnology Center at the Tecnológico de Monterrey in México, where he is currently working in his Ph.D. His early formation was in the Autonomous University of Baja California, where he obtained his title as a Biologist. He then carried out a thesis on the Isolation and Identification of Psychrophillic Organisms from the Antarctica and Expression of a Superoxide Dismutase from Deschampsia antarctica in Escherichia coli.

During the influenza outbreak in 2009, Sergio was part of the team that developed a novel technique for its detection and the creation of a recombinant vaccine against the A/H1N1. He was then invited to the Novartis Fourth Annual Global BioCamp, an event for the biotechnology leaders from the best universities in 27 countries, held at Cambridge, Massachusets in October 2009.


Dr. Manuel I. Zertuche Guerra

Dr. Manuel Zertuche Dr. Manuel Zertuche Guerra has a Masters Degree in Science with a specialization in Production Systems and a Ph.D. in Plant Physiology from Texas A&M. In 1992, he was named the Head of the International Center for Agribusinesses and then became the Director of the Center of Strategic Studies in the Tecnológico de Monterrey. He developed strategic planning projects in Venezuela, Ecuador, Argentina, Bolivia, Nicaragua, and El Salvador. He works as a consultant and has participated in several conferences and seminars in the United States and Canada. He is currently the Head of the Biotechnology and Food Division in the Tecnológico de Monterrey and leads several research projects in collaboration with important Mexican corporations, such as FEMSA, CEMEX, LALA, Tyson, and Landsteiner Scientific.

M. Sc. José Manuel Aguilar Yáñez

M. Sc. José Manuel Aguilar José Manuel Aguilar majored in Biology with a specialty in Molecular Biology at the Baja California Autonomous University. In 2007, he got a M.S. Degree in Biotechnology at the Tecnológico de Monterrey, where he was awarded an Honorary Mention for Excellence and an Outstanding Thesis Mention. He has two scientific publications and two more under revision, as well as five national patent requests and one Patent Cooperation Treaty. As of now, he works at the Tecnológico de Monterrey as a professor and is on his way to finish his Ph.D.

M. Sc. Johari Salgado Gallegos

M. Sc. Johari Salgado Johari Salgado is a biologist graduated from the Baja California Autonomous University. In 2007, he received an M.S. Degree in Biotechnology at the Tecnológico de Monterrey. He participated in the development of an Influenza vaccine during the A/H1N1 2009 outbreak and currently leads several projects in collaboration with Landsteiner Scientific, in addition to teaching the Genetic Engineering Laboratory in the Tecnológico de Monterrey.

Felipe López Pacheco

Felipe López Felipe López Pacheco has a technical degree in Biotechnology and is currently majoring in Biotechnology Engineering. As part of a joint project between the Landsteiner Pharmaceutical Group and ITESM, he is in charge of performing ELISAS to find antibodies.






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