Team:Newcastle/Meetings/26 May 2010

BioBrick 1 (CaCO3/ Urease)
- BioBrick tutorial taught last to last week by Anil on Urease. Urease has a complicated genetic circuit. Should start forming questions which would help in building the genetic circuit of the BioBrick.

BioBrick 2 (End of crack and signalling system)
- Need to search for the peptides which are to be used for quorum sensing.

BioBrick 3 (Non target environment kill switch)
- There are not many BioBricks in the iGEM registry on bacterial lysis. There is a lot of data on lysis by Richard on the wiki. WE should start looking up for ftsZ and SOS system for lysis and a BioBrick can be made early based on this and can be characterized. Need to look up on ftsZ paper. Bacterial Time Bomb.

BioBrick 4 (Spider silk & filamentous cells)
- Read the 2 papers sent by Anil on glue and spider silk.

BioBrick 5 (glue and lysis)
- No progress made due to focus on other BioBricks

BioBrick 6 Sequential Programming
- Modelling is almost at an end. (Richard)

Visa/Passport update

Harsh called but didn't get any dates for the month of June so will be applying in July again. Send visa draft document to Anil- Harsh

Travel plans

Too early to book flights and hotels. May have other members joining.

Tutorial

Formal demonstration of Clone Manager next week - Anil

Flow Chart

Flowchart
There are 3 sensing systems:

Are the cells at an optimal density?
Sensing system for glue production
End of crack signalling

Timeline (Janetta)

Put timeline up on the current wiki.

Other business

None

Action points

Send the email to Colin Harwood (Richard)
Someone ask Anil to put the powerpoint presentation of the tutorial in the dropbox
Booking for the rooms - Jen
Rolling booking has been made for CBCB building for every Wednesday - Anil
New biobricks have arrived
Wendy a post doc and a molecular bilogist would be working with us in the lab and would be guiding us.
Lab training will start from the end of June- Anil
For the lab trainig the current team will be split into 2 teams and the training would last for 2 weeks where

in the 1st week one team would be undergoing their training and the other team would be designing biobricks and in the 2nd team the tasks will be switched between the 2 teams.

Need to plan out what the other team would exactly be doing within that 1 week.
Before the end of June we would be making the design of the biobrick.
Google up for the video on the tutrial for Cell Designer and Kapasi.
Good News: Goksel is now an official advisor of the iGEM team 2010 and would be helping us with modelling

and designing the biobricks.

Need to look up on the registry for the biobricks which are used before, which are new and which one needs optimizing- everyone.
Move Da from biobrick 2 to biobrick 3.
Add Goksel to iGEM 2010 list - Jen.

Next formal meeting

Thursday, 3rd June at 3.00 p.m. at CBCB
Chair: Harsh, Minutes: Rachel, Computer: Jannetta

@@ Line 1: / Line 1: @@
-=Formal Meeting 26th May 2010=
+{{Team:Newcastle/mainbanner}}
-{|
+==Formal Meeting - 26th May 2010==
-| Minutes for formal meeting 1st April 2010
-|-
+*Minutes for formal meeting 1st April 2010
- |* Room: Claremont 922; Start: 3 pm
+* Room: Claremont 922; Start: 3 p.m.
-|-
+* Chair: Rachel, Minutes: Harsh, Computer: Steve
-    |* Chair: Rachel, Minutes: Harsh, Computer: Rachel
+* [[Team:Newcastle/Agendas/26_May_2010|Agenda]]
-|-
-   |1. Roll call and apologies
+====Roll call and apologies====
-|-
-|Apologies - Steven, Younus, Da, Alan, Phil
+* Absent: Steven, Younus, Da, Alan, Phil
-|-
-  | 2. Approval of agenda and minutes
+====Approval of agenda and minutes====
-|-
+* Approved
-|Approved
-|-
+====Brief summary of previous meeting====
-   |3. Brief summary of previous meeting
+* Run the demo tutorial -  Richard, Jannetta, Steven
-|- Run the demo tutorial- Richard, Jannetta, Steven
-  | 4. Research Feedback
+====Research Feedback====
-|-
-         |1. Biobrick 1 (CaCO3/ Urease)
+* '''BioBrick 1 (CaCO3/ Urease)'''
-Biobrick tutorial taught last to last week by Anil on Urease. Urease has a complicated genetic circuit. Should start forming questions which would help in building the egnetic circuit of the biobrick.
+**BioBrick tutorial taught last to last week by Anil on Urease. Urease has a complicated genetic circuit. Should start forming questions which would help in building the genetic circuit of the BioBrick.
-|-
-         |2. Biobrick 2 (End of crack and signalling system)
+* '''BioBrick 2 (End of crack and signalling system)'''
-Need to search for the peptides which are to be used for quorum sensing.
+**Need to search for the peptides which are to be used for quorum sensing.
-|-
-         |3. Quorum Sensing
+* '''BioBrick 3 (Non target environment kill switch)'''
-Number of competence cells not an issue. Sin operone to control a lot of pathway.
+**There are not many BioBricks in the iGEM registry on bacterial lysis. There is a lot of data on lysis by Richard on the wiki. WE should start looking up for ftsZ and SOS system for lysis and a BioBrick can be made early based on this and can be characterized. Need to look up on ftsZ paper. Bacterial Time Bomb.
-|-
-         |4. Other points
+* '''BioBrick 4 (Spider silk & filamentous cells)'''
-A. FtsZ knockout to allow filamentous growth, thereby forming a mesh with glue either secreted out or remains in the cells until lysis.
+**Read the 2 papers sent by Anil on glue and spider silk.
-B. Is spider silk able to resist the alkali conditions?
-C. Is it possible to produce spider silk in Bacillus?
+* '''BioBrick 5 (glue and lysis''')
-|-
+**No progress made due to focus on other BioBricks
-  | 5. Voigt paper feedback
-Salmonella to secrete spider silk contained in SPI through T3SS. SPI to contain ADF1, ADF2 and ADF3 genes. Does Bacillus have a T3SS? Can we allow proteins to fold in the cells then induce lysis? To check out Bacillus secretion system.
+* '''BioBrick 6 Sequential Programming'''
-|-
+**Modelling is almost at an end. (Richard)
-  | 6. Visa/Passport update
-Harsh to apply on May, June period
+====Visa/Passport update====
-|-
+Harsh called but didn't get any dates for the month of June so will be applying in July again.
-  | 7. Travel plans
+Send visa draft document to Anil- Harsh
-Too early to book flights and hotels. May have other members joining.
-|-
+====Travel plans====
-  | 8. Tutorial
+* Too early to book flights and hotels. May have other members joining.
-Next tutorial to be on the week after
-|-
+====Tutorial====
-  | 9. Flow Chart
+* Formal demonstration of Clone Manager next week - Anil
-[[Media:Flowchart_overview-1-.ppt‎ ]]
-A. Need to be more in depth. ie. The problems, what the bacteria need to do to achieve the goal and the genes that are needed and why.
+====Flow Chart====
-B. Another flow chart to show the different stituation and how the bacteria response to it.
+* [[Media:Flowchart_overview-1-.ppt‎|Flowchart]]
-|-
+* There are 3 sensing systems:
-  | 10. Sponser on T-shirt (Janetta)
+# Are the cells at an optimal density?
-To contact sponser next week
+# Sensing system for glue production
-|-
+# End of crack signalling
-  | 11. Other business
-A. Foxit not working
+====Timeline (Janetta)====
-|-
+* Put timeline up on the current wiki.
-  | 12. Action points
-. To draft email to Colin and Chris and show it to instructors first(everyone)
+====Other business====
-. To continue to develop flow chart(everyone)
+* None
-. Tutorial on Wiki
-|-
+====Action points====
-   |14. Next meeting 11am on 070410 for informal meeting in Walton library and 3pm on 070410 for formal meeting in CBCB
-. Chair-Richard
+* Send the email to Colin Harwood (Richard)
-. Minutes-Racheal
+* Someone ask Anil to put the powerpoint presentation of the tutorial in the dropbox
-. Computer-Jenetta
+* Booking for the rooms - Jen
-|-
+* Rolling booking has been made for CBCB building for every Wednesday - Anil
+* New biobricks have arrived
+* Wendy a post doc and a molecular bilogist would be working with us in the lab and would be guiding us.
+* Lab training will start from the end of June- Anil
+* For the lab trainig the current team will be split into 2 teams and the training would last for 2 weeks where
+in the 1st week one team would be undergoing their training and the other team would be designing biobricks and
+in the 2nd team the tasks will be switched between the 2 teams.
+* Need to plan out what the other team would exactly be doing within that 1 week.
+* Before the end of June we would be making the design of the biobrick.
+* Google up for the video on the tutrial for Cell Designer and Kapasi.
+* Good News: Goksel is now an official advisor of the iGEM team 2010 and would be helping us with modelling
+and designing the biobricks.
+*  Need to look up on the registry for the biobricks which are used before, which are new and which one needs optimizing- everyone.
+* Move Da from biobrick 2 to biobrick 3.
+* Add Goksel to iGEM 2010 list - Jen.
+====Next formal meeting====
+*Thursday, 3rd June at 3.00 p.m. at CBCB
+*Chair: Harsh, Minutes: Rachel, Computer: Jannetta
+{{Team:Newcastle/footer}}

Team:Newcastle/Meetings/26 May 2010

From 2010.igem.org

Latest revision as of 09:16, 27 October 2010

Contents

Formal Meeting - 26th May 2010

Roll call and apologies

Approval of agenda and minutes

Brief summary of previous meeting

Research Feedback