"
Team:Newcastle/Meetings/4 June 2010
From 2010.igem.org
(Difference between revisions)
(→LAB TRAINING GROUPS:) |
(→Informal Meeting - 4 June 2010) |
||
| Line 30: | Line 30: | ||
* [http://www.youtube.com/watch?v=FRijFX0BpGI&feature=player_embedded Video for lego pipette] | * [http://www.youtube.com/watch?v=FRijFX0BpGI&feature=player_embedded Video for lego pipette] | ||
| - | ====1st lab week==== | + | ====1st lab week==== |
* RC | * RC | ||
* DY | * DY | ||
* AK | * AK | ||
* (HS) | * (HS) | ||
| - | + | (Others modelling Filamentous and spidersilk killswitch) | |
| - | ====2nd lab week==== | + | |
| + | ====2nd lab week==== | ||
*JS | *JS | ||
*RB | *RB | ||
| Line 43: | Line 44: | ||
*(YE) | *(YE) | ||
*Urease both | *Urease both | ||
| + | (Others Modelling glue and lysis quorum sensing) | ||
====LAB TRAINING GROUPS:==== | ====LAB TRAINING GROUPS:==== | ||
Revision as of 08:56, 27 October 2010
| |||||||||||||
| |||||||||||||
Contents |
Informal Meeting - 4 June 2010
Urease tutorial-recap
- Accessory genes and nickel incorporation necessary more complicated than we thought.
- Software engineering cycle
- Requirements -> specification-> design-> implementation -> testing -> maintenance ...link back to the requirements
- Specification more detail on the requirements- literature
- Urease calcium carbonate, optimise for Bacillis
- Increase expression- what we thought!
- Biosynthesis of active BS in the Absence of Known Urease Accessory Proteins: paper
- Accessory genes required!
- Look over papers from tutorial
- Sporosarcina pasteurii
- Biobrick site symbols
- Promoter-ribosome- binding- coding sequence –ribosome binding site-coding-ribosome binding coding- terminator sequences ...and spacers
- pSpac
- ncbi – find the genome – can see the genes
- Implementation using sequencher
- Find the sequence of part
- Assemble parts
- Biobrick friendly restriction sites
- Genbank/embl file format
- Implementation altered by cloning strategy
- Biobricks RFC- request for comments
- [http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_10:_Draft_standard_for_Biobrick_biological_parts duplo and lego don’t fit together]
- [http://www.youtube.com/watch?v=FRijFX0BpGI&feature=player_embedded Video for lego pipette]
1st lab week
- RC
- DY
- AK
- (HS)
(Others modelling Filamentous and spidersilk killswitch)
2nd lab week
- JS
- RB
- SW
- PH
- (YE)
- Urease both
(Others Modelling glue and lysis quorum sensing)
LAB TRAINING GROUPS:
- Group 1 starts 14th June: SW, D.Y AK, (HS); working on Biobrick 2 & 5 (End of crack & signalling system; Glue & lysis)
- Group 2 starts 21st June: JS, RB, RC, PH, (YE); working on BioBrick 3 & 4 (Non-target-environment kill switch; Spider silk and filamentous cells)
- All team members working on BioBrick 1 (CaCO3/Urease).
- RC working on BioBrick 6 (sequential programming)
- Team members not in the lab training will be working on modeling.
- HS and YE will unfortunately be away for both lab training sessions, hence the brackets
   
|
    
|
