Team:Alberta/Notebook/protocols/gel extraction

From 2010.igem.org

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==General Protocols==
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-----------------------------
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*[[Team:Alberta/Notebook/protocols/invitro_biobyte_assembly | In Vitro BioByte Assembly]]
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-----------------------------
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*[[Team:Alberta/Notebook/protocols/LB | LB Plates and Broth]]
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*[[Team:Alberta/Notebook/protocols/transformations |Transformations]]
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*[[Team:Alberta/Notebook/protocols/overnight |5mL Overnight ]]
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*[[Team:Alberta/Notebook/protocols/glycerol | Glycerol Stock ]]
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-----------------------------
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*[[Team:Alberta/Notebook/protocols/miniprep | Plasmid Miniprep ]]
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*[[Team:Alberta/Notebook/protocols/digest | Restriction Digest ]]
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*[[Team:Alberta/Notebook/protocols/vector_dephos | Vector Dephosphorylation]]
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*[[Team:Alberta/Notebook/protocols/ligation | Ligation ]]
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-----------------------------
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*[[Team:Alberta/Notebook/protocols/agarose_gel | Agarose Gel Electrophoresis ]]
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*[[Team:Alberta/Notebook/protocols/gel_extraction | Gel Extraction ]]
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-----------------------------
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*[[Team:Alberta/Notebook/protocols/pcr | PCR]]
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*[[Team:Alberta/Notebook/protocols/colony_pcr | Colony PCR ]]
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*[[Team:Alberta/Notebook/protocols/pcr_purification | PCR Purification ]]
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-----------------------------
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*[[Team:Alberta/Notebook/protocols/labelling | Sample Labelling Conventions]]
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*[[Team:Alberta/Notebook/protocols/sequencing | Fluorescent Sequencing Reaction]]
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*[[Team:Alberta/Notebook/protocols/primer_design | Primer Design]]
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-----------------------------
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Latest revision as of 02:29, 27 October 2010

TEAM ALBERTA


Gel Extraction

Reagents:

  • Clean scalpel
  • Transilluminator
  • Eppendorf tube
  • Gel extraction kit
  • 55oC water bath
  • Vortex
  • Microfuge


Procedure:

  • Place the gel on the transilluminator. Put on face shield and gloves.
  • Turn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
  • Pry out the gel ‘cube’ and place it in an eppendorf tube.
  • Follow instructions on pp. 25 of [http://www.qiagen.com/literature/render.aspx?id=103715 QIAQuick Spin Handbook].


Retrieved from "http://2010.igem.org/Team:Alberta/Notebook/protocols/gel_extraction"