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Team:Alberta/Notebook/protocols/glycerol
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| - | {{Team:Alberta/navbar| | + | {{Team:Alberta/navbar|notebook=selected}} |
| + | |||
| + | {{Team:Alberta/beginLeftSideBar|toc=NO}} | ||
| + | |||
| + | |||
| + | ==General Protocols== | ||
| + | ----------------------------- | ||
| + | *[[Team:Alberta/Notebook/protocols/invitro_biobyte_assembly | In Vitro BioByte Assembly]] | ||
| + | ----------------------------- | ||
| + | *[[Team:Alberta/Notebook/protocols/LB | LB Plates and Broth]] | ||
| + | |||
| + | *[[Team:Alberta/Notebook/protocols/transformations |Transformations]] | ||
| + | |||
| + | *[[Team:Alberta/Notebook/protocols/overnight |5mL Overnight ]] | ||
| + | |||
| + | *[[Team:Alberta/Notebook/protocols/glycerol | Glycerol Stock ]] | ||
| + | ----------------------------- | ||
| + | *[[Team:Alberta/Notebook/protocols/miniprep | Plasmid Miniprep ]] | ||
| + | |||
| + | *[[Team:Alberta/Notebook/protocols/digest | Restriction Digest ]] | ||
| + | |||
| + | *[[Team:Alberta/Notebook/protocols/vector_dephos | Vector Dephosphorylation]] | ||
| + | |||
| + | *[[Team:Alberta/Notebook/protocols/ligation | Ligation ]] | ||
| + | ----------------------------- | ||
| + | *[[Team:Alberta/Notebook/protocols/agarose_gel | Agarose Gel Electrophoresis ]] | ||
| + | |||
| + | *[[Team:Alberta/Notebook/protocols/gel_extraction | Gel Extraction ]] | ||
| + | ----------------------------- | ||
| + | *[[Team:Alberta/Notebook/protocols/pcr | PCR]] | ||
| + | |||
| + | *[[Team:Alberta/Notebook/protocols/colony_pcr | Colony PCR ]] | ||
| + | |||
| + | *[[Team:Alberta/Notebook/protocols/pcr_purification | PCR Purification ]] | ||
| + | ----------------------------- | ||
| + | *[[Team:Alberta/Notebook/protocols/labelling | Sample Labelling Conventions]] | ||
| + | |||
| + | *[[Team:Alberta/Notebook/protocols/sequencing | Fluorescent Sequencing Reaction]] | ||
| + | |||
| + | *[[Team:Alberta/Notebook/protocols/primer_design | Primer Design]] | ||
| + | ----------------------------- | ||
| + | |||
| + | |||
| + | {{Team:Alberta/endMainContent}} | ||
| + | |||
| - | |||
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| - | Protocol | + | ==Protocol: Glycerol Stock== |
| - | Reagents: | + | ===Reagents:=== |
* Overnight with bacterial growth (probably 1.5mL) | * Overnight with bacterial growth (probably 1.5mL) | ||
| Line 21: | Line 64: | ||
| - | Procedure: | + | ===Procedure:=== |
* Pipet 150uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes. | * Pipet 150uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes. | ||
| Line 30: | Line 73: | ||
| - | Notes: | + | ===Notes:=== |
* If we make extra we have them if we need to send them out or want to store in several locations. | * If we make extra we have them if we need to send them out or want to store in several locations. | ||
| - | + | ||
{{Team:Alberta/endMainContent}} | {{Team:Alberta/endMainContent}} | ||
Latest revision as of 02:21, 27 October 2010
Protocol: Glycerol Stock
Reagents:
- Overnight with bacterial growth (probably 1.5mL)
- Screw cap tubes
- Glycerol
Procedure:
- Pipet 150uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes.
- Add 350uL of overnight culture to each tube.
- Pipet up and down to gently mix.
- Flash freeze in liquid N2 or dry ice/methanol bath.
- Place in -80oC freezer when frozen.
Notes:
- If we make extra we have them if we need to send them out or want to store in several locations.
