Revision as of 01:23, 26 October 2010

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Problem

Sending off yneA BioBrick

Aim

The aim of this experiment is to extract ligated yneA fragment in the plasmid pSB1C3 from E. coli cells and purify them and freeze dry them to send them off to the iGEM headquarters.

Materials and Protocol

Please refer to: Qiagen Minipreps for plasmid extraction from E. coli cells.

Please refer to: Nanodrop Spectrophotometer to check the purity of the extracted and purified plasmid.

Result

Tube	Nanodrop Value (in ng/ml)
1	158.7
2	261.2
3	262.9
4	204.7
5	262.9
6	202.1
7	174.8
8	226.6
9	229.1
10	164.5
11	177.8
12	225.1
13	281.5
14	249.3
15	192.2
16	239.3

Arabinose-inducible promoter characterisation

...

Miniprep of BBa_I13401 (GFP coding sequence + terminator)

..from last night's overnight cultures from the 8th of september transformation.

Double digests

.. double digests for standard BioBrick assembly.. cut the arabinose-inducible promoter BioBrick with EcoRI and SpeI, cut gfp+terminator BioBrick with EcoRI and XbaI, for later ligation..

@@ Line 21: / Line 21: @@
 |-
 |1
-|76.5
+|158.7
 |-
 |2
-|61.5
+|261.2
 |-
 |3
-|84.6
+|262.9
 |-
 |4
-|19.5
+|204.7
 |-
 |5
-|89.9
+|262.9
 |-
 |6
-|87.6
+|202.1
 |-
 |7
-|6.3
+|174.8
 |-
 |8
-|69.5
+|226.6
 |-
 |9
-|81.3
+|229.1
 |-
 |10
-|74.4
+|164.5
 |-
 |11
-|68.5
+|177.8
 |-
 |12
-|70.2
+|225.1
 |-
 |13
-|83.5
+|281.5
 |-
 |14
-|77.6
+|249.3
 |-
 |15
-|73.3
+|192.2
 |-
 |16
-|63.0
+|239.3
 |}
-Nanodrop results for pSB1C3 + ''yne''A:
-#158.7
-#261.2
-#262.9
-#204.7
-#262.9
-#202.1
-#174.8
-#226.6
-#229.1
-#164.5
-#177.8
-#225.1
-#281.5
-#249.3
-#192.2
-#239.3
-#195.1
 =Arabinose-inducible promoter characterisation=

Team:Newcastle/10 September 2010

From 2010.igem.org