Team:Newcastle/Meetings/6 October 2010

From 2010.igem.org

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=6 October 2010 Meeting=
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==Formal Meeting - 6 October 2010==
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==Roll call==
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* Apologies: Colin Harwood, Jem and Phil
 +
* [[Team:Newcastle/Agendas/6_October_2010#Agenda_for_the_formal_meeting:_Wednesday__6th_October_2010 | Agenda]]
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* Apologies: Colin Harwood and Jem
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===Presentation===
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==Presentation==
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=====Introduction=====
-
===Bacilla Filla slide===
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* Mention why do we use this bug and that it is able to live in high pH
 +
* Have to menetion that we all biobrick have been modelled by SBML and copasi
 +
 
 +
=====Bacilla Filla slide=====
* The solution words to be smaller than the logo
* The solution words to be smaller than the logo
* Logo have to be the biggest!
* Logo have to be the biggest!
* Solution at the top of the page with a small logo below it then zoom into the logo
* Solution at the top of the page with a small logo below it then zoom into the logo
-
===Technical review slide===
+
=====Technical review slide=====
* Show the link between the genes
* Show the link between the genes
* Some of the summary slide's information to go here, example: swim down the cracks, the tent, spray on, etc.
* Some of the summary slide's information to go here, example: swim down the cracks, the tent, spray on, etc.
* A single shot overview then to the biobricks
* A single shot overview then to the biobricks
-
* SAY: This is a crack, and here is our bug on the surface of the crack, etc
+
* Say: This is a crack, and here is our bug on the surface of the crack, etc
* Label the pictures!
* Label the pictures!
* Animation for the overview, ie, the background to remain the same
* Animation for the overview, ie, the background to remain the same
-
===Swarming slide===
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=====Swarming slide=====
* Change the flagella of the bug
* Change the flagella of the bug
* Zoom into the plates
* Zoom into the plates
-
===Subtilin slide===
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=====Subtilin slide=====
* Show subtilin only when the bug is at the end of the crack
* Show subtilin only when the bug is at the end of the crack
* Fill up the crack with bugs
* Fill up the crack with bugs
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* Change the pveg promoter to the oxygen sensitive promoter
* Change the pveg promoter to the oxygen sensitive promoter
-
==Lab feedback==
+
=====Subtilin immunity slide=====
-
===Filamentous cells===
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* SAY: Subtilin immunity already in the registry, that is why we use it
 +
* Mention what the individual CDS do
 +
* Green tick for those that we did and a red cross for those that we did not
 +
 
 +
=====Workflow slide=====
 +
* Change the word interesting to valuable to all synthetic biologist
 +
* Cut down on the script
 +
* Have to make it fit to the presentation
 +
* Integration of lots of data that is why we use e-science
 +
 
 +
=====YneA slide=====
 +
* Put filamentous cells into the pictures
 +
* Metabolic pathway picture and link it
 +
 
 +
=====Glue slide=====
 +
* The iGEM plates to go onto it
 +
 
 +
=====Kill switch=====
 +
* Can bin it
 +
* Move to the ethic page
 +
 
 +
=====Ethic slide=====
 +
* Move it to the front after the introduction and also mention in the summary
 +
* What is the danger and how we make it safe
 +
 
 +
=====Others=====
 +
* Show that the bug is able to grow in concrete
 +
 
 +
=====Urease slide=====
 +
* Have to show that calcium carbonate is filling up the cracks
 +
* Have to say that we are better than the rest
 +
* Highlight the advantage of ours and the disadvantage of others
 +
* Mention SR1
 +
 
 +
===Lab feedback===
 +
=====Filamentous cells=====
* Redo the microscopic  
* Redo the microscopic  
* Correlate between the expression of GFP and the length
* Correlate between the expression of GFP and the length
-
===SR1===
+
=====SR1=====
* We have colonies
* We have colonies
-
===Scanning electron microscopy===
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=====Scanning electron microscopy=====
* The concrete samples are already with the EM unit
* The concrete samples are already with the EM unit
-
==Agenda==
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===Agenda===
-
* To reedit the agenda
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* To re-edit the agenda
-
==Action points==
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===Action points===
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* '''Rachel''' and '''Phil''': Characterisation data for filamentous cells brick needs to be put on the wiki and the parts registry.
+
* Rachel and Phil: Characterisation data for filamentous cells brick needs to be put on the wiki and the parts registry.
* Everyone to do up the presentation slides first.
* Everyone to do up the presentation slides first.
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==Next meeting==
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===Next meeting===
-
Wed 4pm.
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*13th, Wed October, 4 p.m.
-
 
+
*Chair: Steven, Computer: Alan, Minutes: Deena
-
*'''Chair:''' Steven
+
-
*'''Computer:''' Alan
+
-
*'''Minutes:''' Deena
+

Latest revision as of 01:10, 26 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Formal Meeting - 6 October 2010

  • Apologies: Colin Harwood, Jem and Phil
  • Agenda

Presentation

Introduction
  • Mention why do we use this bug and that it is able to live in high pH
  • Have to menetion that we all biobrick have been modelled by SBML and copasi
Bacilla Filla slide
  • The solution words to be smaller than the logo
  • Logo have to be the biggest!
  • Solution at the top of the page with a small logo below it then zoom into the logo
Technical review slide
  • Show the link between the genes
  • Some of the summary slide's information to go here, example: swim down the cracks, the tent, spray on, etc.
  • A single shot overview then to the biobricks
  • Say: This is a crack, and here is our bug on the surface of the crack, etc
  • Label the pictures!
  • Animation for the overview, ie, the background to remain the same
Swarming slide
  • Change the flagella of the bug
  • Zoom into the plates
Subtilin slide
  • Show subtilin only when the bug is at the end of the crack
  • Fill up the crack with bugs
  • Describle the subtilin signalling system
  • Change the pveg promoter to the oxygen sensitive promoter
Subtilin immunity slide
  • SAY: Subtilin immunity already in the registry, that is why we use it
  • Mention what the individual CDS do
  • Green tick for those that we did and a red cross for those that we did not
Workflow slide
  • Change the word interesting to valuable to all synthetic biologist
  • Cut down on the script
  • Have to make it fit to the presentation
  • Integration of lots of data that is why we use e-science
YneA slide
  • Put filamentous cells into the pictures
  • Metabolic pathway picture and link it
Glue slide
  • The iGEM plates to go onto it
Kill switch
  • Can bin it
  • Move to the ethic page
Ethic slide
  • Move it to the front after the introduction and also mention in the summary
  • What is the danger and how we make it safe
Others
  • Show that the bug is able to grow in concrete
Urease slide
  • Have to show that calcium carbonate is filling up the cracks
  • Have to say that we are better than the rest
  • Highlight the advantage of ours and the disadvantage of others
  • Mention SR1

Lab feedback

Filamentous cells
  • Redo the microscopic
  • Correlate between the expression of GFP and the length
SR1
  • We have colonies
Scanning electron microscopy
  • The concrete samples are already with the EM unit

Agenda

  • To re-edit the agenda

Action points

  • Rachel and Phil: Characterisation data for filamentous cells brick needs to be put on the wiki and the parts registry.
  • Everyone to do up the presentation slides first.

Next meeting

  • 13th, Wed October, 4 p.m.
  • Chair: Steven, Computer: Alan, Minutes: Deena