Revision as of 22:27, 25 October 2010

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Problem

Genomic DNA extraction experiment

Aims

The aim of today's experiment is to extract genomic DNA from both B. subtilis strains 168 and 3610. The genes necessary for the swarming BioBrick and rocF BioBrick will then hopefully be obtained from the genomic DNA using PCR.

Protocol

Please refer to: DNA extraction of B. subtilis for materials required and protocol.

Discussion

At the end of the DNA precipitation step, we observed a small white pellet in all the eppendorf tubes.

Conclusion

The experiment was a success! The content and purity of the extracted DNA will be checked by using PCR on 28th July, 2010.

@@ Line 18: / Line 18: @@
 ==Conclusion==
 The experiment was a success! The content and purity of the extracted DNA will be checked by using PCR on 28th July, 2010.
-=Gel Electrophoresis=
-==Aims==
-...
-[[Image:Newcastle_Gel_Extraction_genomic_DNA.jpg|thumb|200px|right]]
-==Protocol==
-* Please refer to the [[Team:Newcastle/Gel electrophoresis|gel electrophoresis]] page for full protocol.
-==Discussion==
-* Unfortunately no bands were observed on the gel.
-==Conclusion==
-As no bands were observed on the gel, the PCR did not work. This could be due to a number of reasons: It could be due to the DNA not being extracted or that we might have used the wrong primers. It could also be caused by the small size of the fragment that we amplified which ran off the gel because we ran the gel for too long.
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Team:Newcastle/27 July 2010

From 2010.igem.org