Team:Newcastle/Meetings/21 October 2010
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**Why bacillus? | **Why bacillus? | ||
**bioconcrete- show its current | **bioconcrete- show its current | ||
- | **Non-pathogenic not non-virulent | + | **Non-pathogenic not non-virulent |
+ | *BioBricks: | ||
**Previous work on CaCO<sub>3</sub> | **Previous work on CaCO<sub>3</sub> | ||
**Don't use the word '''PROVES''' | **Don't use the word '''PROVES''' | ||
- | |||
**Alan - Flux first, speak slowly | **Alan - Flux first, speak slowly | ||
- | + | *Gold: | |
- | + | ||
- | *Gold | + | |
**Jannetta write RFC | **Jannetta write RFC | ||
**number on judging form | **number on judging form | ||
**webservice!!! | **webservice!!! | ||
- | * | + | **Separate slid for work flows |
+ | *SEM: | ||
**Images are ours | **Images are ours | ||
+ | *Summary table | ||
+ | **redo with BRONZE, SILVER, GOLD section?! | ||
==Other== | ==Other== |
Revision as of 16:29, 25 October 2010
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- Apologies - Phil, Younus, Deena late
- Action point: Phil and Rachel add characteristation data to the wiki
Contents |
yneA Characteristaion:
- ΔamyE:Pspac(hy)::yneA
- B. subtilis spread of IPTG may not be homogenous through the population.
- E. coli has transporter deltions: check E. coli wiki
- take out pMap65 results
- Median, Mode add to graph
- pMutin4 proportion of cells
- 20% responding- looks like
- Biomass of cells - length * amount (counts)) against IPTG
- Lysis acts to counter the overexpression of yneA ... so we have fewer cells
- Younus AP: send script for yneA to Jen, Matt, Neil
- Plot: Max cell length against ITPG + Error bars
Arabinose
- We think we have it cloned infront of GFP
- B. subtilis transformation tommorrow
- Fluorescence
- Characterisation of previous part!!!
Concrete
- Damaged building - earthquake
Flux balance
- difference between optimal and normal
Lab work
- DNA submitted - tracking number sent
- 1C3 SR1 has not arrived, we would have had 2 bricks submitted!
Safety page
- Deena and Harsh
- Risk assessment - Bacillus
- BioCOSH
- Safety Guy talk mention it
Presentation
- Phil - DON'T BE AGGRESSIVE!!!!
- Beginning:
- say what the project is about
- mention Bacillus subtilis
- Why bacillus?
- bioconcrete- show its current
- Non-pathogenic not non-virulent
- BioBricks:
- Previous work on CaCO3
- Don't use the word PROVES
- Alan - Flux first, speak slowly
- Gold:
- Jannetta write RFC
- number on judging form
- webservice!!!
- Separate slid for work flows
- SEM:
- Images are ours
- Summary table
- redo with BRONZE, SILVER, GOLD section?!
Other
- Insurance form
- Taxi
- Younus - Poster next thursday due in?
- Action point check presentation and poster submission!!!
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