Team:Newcastle/Meetings/21 October 2010

From 2010.igem.org

(Difference between revisions)
Line 20: Line 20:
20% responding- looks like  
20% responding- looks like  
-
Biomass of cells - length * amount(counts)) against IPTG  
+
Biomass of cells - length * amount (counts)) against IPTG  
Lysis acts to counter the overexpression of yneA ... so we have fewer cells  
Lysis acts to counter the overexpression of yneA ... so we have fewer cells  
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'''Phil''' DON'T BE AGGRESSIVE!!!!
'''Phil''' DON'T BE AGGRESSIVE!!!!
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Begining:  
+
Beginning:  
*say what the project is about  
*say what the project is about  
*mention ''Bacillus subtilis''
*mention ''Bacillus subtilis''
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*Why bacillus  
+
*Why bacillus?
*bioconcrete- show its current
*bioconcrete- show its current
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Separate slid for work flows  
Separate slid for work flows  
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Alan- Flux first, speak slowly
+
Alan - Flux first, speak slowly
Summary table- redo
Summary table- redo
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Taxi  
Taxi  
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Younus Poster!!! next thursday due in?   
+
Younus - Poster next thursday due in?   
Action point check presentation and poster submission!!!
Action point check presentation and poster submission!!!
 +
 +
{{Team:Newcastle/footer}}

Revision as of 16:09, 25 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Apologies - Phil, Younus, Deena late

Action point: Phil and Rachel add characteristation data to the wiki

Contents

yneA Characteristaion:

  • ΔamyE:Pspac(hy)::yneA
  • Bacillus subtilis spread of IPTG may not be homogenous through the population.
  • E.coli has transporter deltions: check E.coli wiki

take out pMap65 results

Median, Mode add to graph

pMutin4 proportion of cells

20% responding- looks like

Biomass of cells - length * amount (counts)) against IPTG

Lysis acts to counter the overexpression of yneA ... so we have fewer cells

Younus AP: send script for yneA to Jen, Matt, Neil

Plot: Max cell length against ITPG + Error bars

Arabinose

We think we have it cloned infront of GFP Bacillus subtilis transformation tommorrow Fluorescence Characterisation of previous part!!!

Concrete

Damaged building- earthquake

Flux balance

difference between optimal and normal

Lab work

DNA submitted... tracking number sent 1C3 SR1 has not arrived, we would have had 2 bricks submitted!

Safety page

Deena and Harsh

Risk assessment- Bacillus

BioCOSH

Safety Guy talk mention it

Presentation

Phil DON'T BE AGGRESSIVE!!!!

Beginning:

  • say what the project is about
  • mention Bacillus subtilis
  • Why bacillus?
  • bioconcrete- show its current

Non-pathogenic not non-virulent Previous work on CaCO3 Don't use the word PROVES Separate slid for work flows

Alan - Flux first, speak slowly

Summary table- redo

BRONZE, SILVER, GOLD!!!!!!!

GOLD

Jannetta write RFC-

  • number on judging form
  • webservice!!!

SEM

Images are ours

Other

Insurance form Taxi

Younus - Poster next thursday due in?

Action point check presentation and poster submission!!!

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