Team:Peking/Modeling
From 2010.igem.org
Line 16: | Line 16: | ||
<font size=3><font color=#000000><font face="Times New Roman"> | <font size=3><font color=#000000><font face="Times New Roman"> | ||
We adopted the process of Reverse Engineering which in our work means to enumerate all possible network topologies and analyzed whether they fit the objection function or not, thus getting the right topology. | We adopted the process of Reverse Engineering which in our work means to enumerate all possible network topologies and analyzed whether they fit the objection function or not, thus getting the right topology. | ||
+ | <br> | ||
<br> | <br> | ||
Here we chose the object function as Input-Output Alignment. In order to define IOA precisely for need of calculation, we considered most important characters of IOA and adopted Pearson Correlation Coefficient r to represent Input-Output Linear Relationship in the overall search work ( when r>0.99 we consider the network topology having the IOA function ), and also, regulated two levels for the initial and ultimate output concentration for the second character – the output range in further search work.(Figure 1) | Here we chose the object function as Input-Output Alignment. In order to define IOA precisely for need of calculation, we considered most important characters of IOA and adopted Pearson Correlation Coefficient r to represent Input-Output Linear Relationship in the overall search work ( when r>0.99 we consider the network topology having the IOA function ), and also, regulated two levels for the initial and ultimate output concentration for the second character – the output range in further search work.(Figure 1) | ||
+ | <br> | ||
<br> | <br> | ||
<img src="https://static.igem.org/mediawiki/2010/9/98/Target_function.jpg" align=middle width="450"> | <img src="https://static.igem.org/mediawiki/2010/9/98/Target_function.jpg" align=middle width="450"> | ||
+ | <br> | ||
<br> | <br> | ||
<b><font size=2>Figure 1 Factors for selection of IOA network topologies.</b> r is the Pearson Correlation Coefficient and the output range is HIGHLEVEL minus LOWLEVEL.</font> | <b><font size=2>Figure 1 Factors for selection of IOA network topologies.</b> r is the Pearson Correlation Coefficient and the output range is HIGHLEVEL minus LOWLEVEL.</font> | ||
Line 28: | Line 31: | ||
<font size=3><font color=#000000><font face="Times New Roman"> | <font size=3><font color=#000000><font face="Times New Roman"> | ||
In this part, we will demostrate our calculating process in three sections--Network enumeration, Equations set up and network topologies’ analysis. | In this part, we will demostrate our calculating process in three sections--Network enumeration, Equations set up and network topologies’ analysis. | ||
+ | <br> | ||
<br> | <br> | ||
| |
Revision as of 10:44, 24 October 2010
Modeling Home
Introduction
We adopted the process of Reverse Engineering which in our work means to enumerate all possible network topologies and analyzed whether they fit the objection function or not, thus getting the right topology.
Here we chose the object function as Input-Output Alignment. In order to define IOA precisely for need of calculation, we considered most important characters of IOA and adopted Pearson Correlation Coefficient r to represent Input-Output Linear Relationship in the overall search work ( when r>0.99 we consider the network topology having the IOA function ), and also, regulated two levels for the initial and ultimate output concentration for the second character – the output range in further search work.(Figure 1)
Figure 1 Factors for selection of IOA network topologies. r is the Pearson Correlation Coefficient and the output range is HIGHLEVEL minus LOWLEVEL.
Calculating Process
In this part, we will demostrate our calculating process in three sections--Network enumeration, Equations set up and network topologies’ analysis.
==To dig further ==
Analyses and Results
As we search for proper network in two ways: linear and semilog, this part is divided into two sections -- linear and semilog.
==To dig further ==