Team:SDU-Denmark/labnotes6
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Methods: | Methods: | ||
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Notes:<br> | Notes:<br> | ||
100 mL ON culture was made, cells were grown at 37 C in LB media with Chloramphenicol. | 100 mL ON culture was made, cells were grown at 37 C in LB media with Chloramphenicol. | ||
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Done by: Marie & Tommy<br> | Done by: Marie & Tommy<br> | ||
Methods: Miniprep, Restriction digest, gel<br> | Methods: Miniprep, Restriction digest, gel<br> | ||
- | Protocols: | + | Protocols: [https://2010.igem.org/Team:SDU-Denmark/protocols#MP1.2 MP1.2], [https://2010.igem.org/Team:SDU-Denmark/protocols#RD1.1 RD1.1]. |
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Notes:<br> | Notes:<br> | ||
50 mL of elution buffer was used. DNA concentrations after pooling were measured on NanoDrop to 206,2 ng/microL. | 50 mL of elution buffer was used. DNA concentrations after pooling were measured on NanoDrop to 206,2 ng/microL. | ||
gel was run on produckt, showing recent miniprep compared to old miniprep produckt, showing uneven lengths. subsequent was performed.<br> | gel was run on produckt, showing recent miniprep compared to old miniprep produckt, showing uneven lengths. subsequent was performed.<br> | ||
- | [[Image:Team-sdu-denmark-Minipreb_product_of_POT2_with_NinaB_1,5_gel_red_loader__-4.jpg | | + | [[Image:Team-sdu-denmark-Minipreb_product_of_POT2_with_NinaB_1,5_gel_red_loader__-4.jpg | 300px ]] |
==== Restriction digest on miniprep produckt (w. EcoRI) ==== | ==== Restriction digest on miniprep produckt (w. EcoRI) ==== | ||
Date: 17/8<br> | Date: 17/8<br> | ||
Done by: Marie & Tommy<br> | Done by: Marie & Tommy<br> | ||
Methods: Restriction digest, gel<br> | Methods: Restriction digest, gel<br> | ||
- | Protocols: | + | Protocols: [https://2010.igem.org/Team:SDU-Denmark/protocols#MP1.2 MP1.2], gel. |
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Notes:<br> | Notes:<br> | ||
Due to the lack of old sample, restriction digest was performed using only 3,5 microL of miniprep produckt. 1,5 microL of H2O was added insted. EcoRI was used<br> | Due to the lack of old sample, restriction digest was performed using only 3,5 microL of miniprep produckt. 1,5 microL of H2O was added insted. EcoRI was used<br> | ||
The digest mix was incubated for 10 min at 37 C. A gel was run showing uncut new, cut new, uncut old and cut old miniprep product.<br> | The digest mix was incubated for 10 min at 37 C. A gel was run showing uncut new, cut new, uncut old and cut old miniprep product.<br> | ||
- | [[Image:Team-sdu-denmark-Minipreb_product_of_POT2_with_NinaB_1,5_gel_red_loader__-5.jpg | | + | [[Image:Team-sdu-denmark-Minipreb_product_of_POT2_with_NinaB_1,5_gel_red_loader__-5.jpg |300px ]] |
=== PCR on POT2 with NinaB (New Primers) === | === PCR on POT2 with NinaB (New Primers) === | ||
Start date: 20/8<br> | Start date: 20/8<br> | ||
Methods: PCR, Gel<br> | Methods: PCR, Gel<br> | ||
- | Protocols: | + | Protocols: [https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP.1.1] |
==== PCR on POT2 with NinaB (New Primers) ==== | ==== PCR on POT2 with NinaB (New Primers) ==== | ||
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Done by: Marie & Tommy<br> | Done by: Marie & Tommy<br> | ||
Methods: ON<br> | Methods: ON<br> | ||
- | protocos: | + | protocos:[https://2010.igem.org/Team:SDU-Denmark/protocols#CP1.1 CP1.1] |
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Notes:<br> | Notes:<br> | ||
The new primers only contain the innermost restriction sites.<br> | The new primers only contain the innermost restriction sites.<br> | ||
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</head> | </head> | ||
<body> | <body> | ||
- | <table style="text-align: left; width: | + | <table style="text-align: left; width: 300px;" border="1" |
cellpadding="2" cellspacing="2"> | cellpadding="2" cellspacing="2"> | ||
<tr> | <tr> | ||
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</head> | </head> | ||
<body> | <body> | ||
- | <table style="text-align: left; width: | + | <table style="text-align: left; width: 300px;" border="1" |
cellpadding="2" cellspacing="2"> | cellpadding="2" cellspacing="2"> | ||
<tr> | <tr> | ||
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The gel picture shows that alle of the temperatures gave results. | The gel picture shows that alle of the temperatures gave results. | ||
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Latest revision as of 22:21, 23 October 2010