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| | |<html><h2>Strategy</h2></html> | | |<html><h2>Strategy</h2></html> |
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| | + | {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5"; |
| | + | |<html> |
| | + | <a href="https://static.igem.org/mediawiki/2010/6/67/IC_Engineering_Design_Cycle.JPG"><img src="https://static.igem.org/mediawiki/2010/6/67/IC_Engineering_Design_Cycle.JPG" width="200"/></a> |
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| | + | |The Engineering Design Cycle |
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| | |<div ALIGN=CENTER> | | |<div ALIGN=CENTER> |
| | {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5"; | | {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5"; |
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| | |We wanted our kit to be as useful as possible | | |We wanted our kit to be as useful as possible |
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| Photos
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Logos
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| Our Imperial College iGEM Logo
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| PARASIGHT
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| Another really cool logo
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The Team
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| The team with advisors Chris and Tom
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| The Imperial College iGEM Team
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| Some team members with SynBio icon Jay Keasling!!!
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| Maddie and a bit of Kirill in labs
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| Maddie in labs again
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| Nick
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| Maddie in labs... AGAIN
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| Kirill, Maddie and Nick
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| Nick (posing?)
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| Piotr focusing, Anita not so much!
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| Piotr and Anita
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| Maddie and Kirill
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| Anita and Ben
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| Nick knows ONE song on the guitar! Wow
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| Anja, Kyasha and Florian
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| Check out Piotr's laser eyes
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Brainstorming
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| Fv-fragment guided input module
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| Ideas on parasite detection
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| Nick's idea on cell population control
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| Nick's idea...
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| Nick's idea...
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| Nick's idea...
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Wet Lab
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Surface Protein
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| Surface Protein Team Logo
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| Assembly Strategy for LytC
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| Gel confirming that DNA (pSB1C3 and pSB1AK3 with BOO14) has been digested properly by PstI and EcoRI.
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| Gel of PCR confirming pSB1C3 has been amplified.
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| Picture estimating the concentration of purified BOO14 and pSB1C3.
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| Colony PCR to detect sucessfully transformed bacteria.
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| 3 PCR reactions to determine the most effective protocol to amplify the CWB.
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| Gel of a diagnostic digest of the vector confirming that the terminator has been inserted.
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| Isolation of the promoter pVEG by gel purification.
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| Determining the ratio of pSB1C3 to pVEG for ligation.
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| Determining the ratio of lytC to pSB1C3 for ligation.
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Vectors
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| Vectors Team Logo
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| Assembly strategy for the AmyE vector
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| Assembly strategy for the PyrD vector
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| K14002 and oligo assembly
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| K143070 and K11064 assembly step
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XylE
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| XylE Team Logo
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| Team XylE
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| The J23101 gene in a biobrick vector containg RFP gene.
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| Midi-prep XylE digestion with xbaI and PstI
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| Gel analysis of XylE, J23101 promoter and pSB1C3 vector samples
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| Catechol assay (before)
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.jpg)
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| Catechol assay (after) - Can you spot the difference?
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| Catechol assay on XylE-trasformed cells in a 96-well plate
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| First Successful Catechol Assay!
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| First Successful Catechol Assay!
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| :)
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| Test confirming that yellow product of XylE enzmymatic reaction is leaks back from the cytosol into the solution.
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| The coloured pigment is transported out of the cells.
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| Spectra of XylE transformed E.coli after addition of catechol assay
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| Catechol Assay cuvette setup
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| Catechol Assay cuvette setup
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Strategy
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| The Engineering Design Cycle
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| The full assembly strategy
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| Wolf's primers
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Parts
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| BioBricks
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| ComE binding sites in the promoters of the comCDE and comAB operons
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Detection Module
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| Dr Martha Betson explaining the Schistosoma lifecycle
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| Detection Module Construct
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Signalling Module
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| Results of a BLAST search using ComE (Accession no. AAC44897)
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| Results of a BLAST search using BlpR (Accession no. CAC03515.1)
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| Signalling Module Construct
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| Signalling Module Pathway
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Fast Response Module
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| Fast Response Module Construct
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| Fast Response Module Testing Construct
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Human Practices
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| Human Practices panel: advisors
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| The team looks reeeeaaaalllly interested
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| Maddie's notes from the HP panel
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| Notes...
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| Notes...
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| Should scientists be allowed to create artificial life?
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| Should scientists be allowed to create artificial life?
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| Building prototypes informed our design
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| We wanted our kit to be as useful as possible
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School Workshops
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| Brainstorming
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| Chameleon: Now you see me - now you don't
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| More brainstorming
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| ...and more brainstorming
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| Ideas on regulation of Synthetic Biology
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| H'n'H sauce (Hot&Healthy) - yummy
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| More ideas on regulations of SynBio
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| Brainstorming again
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| iGEM <3
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| Check out the lens creating bacteria!
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| More ideas...
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| Happy Bacteria?!
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| Regulations...
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"
