Photos
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Logos
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Our Imperial College iGEM Logo
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PARASIGHT
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Another really cool logo
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The Team
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The team with advisors Chris and Tom
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The Imperial College iGEM Team
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Some team members with SynBio icon Jay Keasling!!!
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Maddie and a bit of Kirill in labs
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Maddie in labs again
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Nick
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Maddie in labs... AGAIN
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Kirill, Maddie and Nick
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Nick (posing?)
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Piotr focusing, Anita not so much!
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Piotr and Anita
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Maddie and Kirill
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Anita and Ben
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Nick knows ONE song on the guitar! Wow
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Anja, Kyasha and Florian
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Check out Piotr's laser eyes
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Brainstorming
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Fv-fragment guided input module
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Ideas on parasite detection
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Nick's idea on cell population control
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Nick's idea...
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Nick's idea...
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Nick's idea...
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Wet Lab
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Surface Protein
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Surface Protein Team Logo
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Assembly Strategy for LytC
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Gel confirming that DNA (pSB1C3 and pSB1AK3 with BOO14) has been digested properly by PstI and EcoRI.
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Gel of PCR confirming pSB1C3 has been amplified.
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Picture estimating the concentration of purified BOO14 and pSB1C3.
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Colony PCR to detect sucessfully transformed bacteria.
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3 PCR reactions to determine the most effective protocol to amplify the CWB.
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Gel of a diagnostic digest of the vector confirming that the terminator has been inserted.
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Isolation of the promoter pVEG by gel purification.
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Determining the ratio of pSB1C3 to pVEG for ligation.
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Determining the ratio of lytC to pSB1C3 for ligation.
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Vectors
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Vectors Team Logo
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Assembly strategy for the AmyE vector
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Assembly strategy for the PyrD vector
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K14002 and oligo assembly
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K143070 and K11064 assembly step
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XylE
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XylE Team Logo
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Team XylE
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The J23101 gene in a biobrick vector containg RFP gene.
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Midi-prep XylE digestion with xbaI and PstI
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Gel analysis of XylE, J23101 promoter and pSB1C3 vector samples
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Catechol assay (before)
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Catechol assay (after) - Can you spot the difference?
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Catechol assay on XylE-trasformed cells in a 96-well plate
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First Successful Catechol Assay!
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First Successful Catechol Assay!
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:)
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Test confirming that yellow product of XylE enzmymatic reaction is leaks back from the cytosol into the solution.
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The coloured pigment is transported out of the cells.
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Spectra of XylE transformed E.coli after addition of catechol assay
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Catechol Assay cuvette setup
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Catechol Assay cuvette setup
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Strategy
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The full assembly strategy
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Wolf's primers
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Parts
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BioBricks
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ComE binding sites in the promoters of the comCDE and comAB operons
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Detection Module
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Dr Martha Betson explaining the Schistosoma lifecycle
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Detection Module Construct
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Signalling Module
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Results of a BLAST search using ComE (Accession no. AAC44897)
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Results of a BLAST search using BlpR (Accession no. CAC03515.1)
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Signalling Module Construct
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Fast Response Module
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Fast Response Module Construct
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Fast Response Module Testing Construct
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Human Practices
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Human Practices panel: advisors
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The team looks reeeeaaaalllly interested
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Maddie's notes from the HP panel
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Notes...
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Notes...
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Should scientists be allowed to create artificial life?
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Should scientists be allowed to create artificial life?
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Building prototypes informed our design
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We wanted our kit to be as useful as possible
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