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Team:TU Delft/Project/sensing/characterization
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==Characterization of the Sensing Parts== | ==Characterization of the Sensing Parts== | ||
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Revision as of 14:21, 22 October 2010
Characterization of the Sensing Parts
Characterized Strains
Strains:
- PalkS / AlkS: E.coli K12/310C
- PalkS / AlkS / PalkB: E.coli K12/311C
- P(CaiF) / AlkS / PalkB: E.coli K12/312C
- Negative control: E.coli K12
The characterization process of the hydrocarbon sensing sub-project will entail output measurements of fluorescence at varying octane concentrations (0.1% – 1%) of the above mentioned strains. These measurements will be performed using a 96-well plate reader. Growth curves will be followed by OD-600 alongside the fluorescence measurements. The obtained fluorescence and growth measurements will be analyzed and modeled accordingly.
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