Team:TU Delft/Project/achievements

From 2010.igem.org

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''Since the standard 3-way ligation protocol gave us a lot of trouble, we came up with a new protocol for the construction of BioBricks. The protocol is basically a 2-way ligation, in which you leave the backbone of the recieving plasmid intact.''
''Since the standard 3-way ligation protocol gave us a lot of trouble, we came up with a new protocol for the construction of BioBricks. The protocol is basically a 2-way ligation, in which you leave the backbone of the recieving plasmid intact.''
** characterization of BioBrick Parts or Devices
** characterization of BioBrick Parts or Devices
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''For the characterization of the [[Team:TU_Delft/Project/solubility/characterization|emulsifier]] and the [[Team:TU_Delft/Project/alkane-degration/characterization|alkane degradation]] biobricks, we developed our own assays.''  
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''For the characterization of the [[Team:TU_Delft/Project/solubility/characterization|emulsifier]] and the [[Team:TU_Delft/Project/alkane-degradation/characterization|alkane degradation]] biobricks, we developed our own assays.''  
** analysis, modeling, and simulation of BioBrick Parts or Devices
** analysis, modeling, and simulation of BioBrick Parts or Devices
''Our team made a new bio-informatics tool for the prediction of protein interactions based on homology, called [[Team:TU_Delft/Modeling/interaction-mapping|HIM]].
''Our team made a new bio-informatics tool for the prediction of protein interactions based on homology, called [[Team:TU_Delft/Modeling/interaction-mapping|HIM]].

Revision as of 13:10, 22 October 2010

Achievements

On this page we would like to elaborate our judgment form a bit more. Of course you can also read our official judgement form.

Requirements for a Bronze Medal:

The whole iGEM project has been awesome. From the moment that we first got together, designed our project untill the stressful final hours before the deadline it was a lot of fun.

  • Successfully complete and submit this iGEM 2010 Judging form.
  • Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.
  • Plan to present a Poster and Talk at the iGEM Jamboree.
  • Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Standard Biological Parts.
  • Submit DNA for at least one new BioBrick Part or Device to the Registry.

Requirements for a Silver Medal:

  • Demonstrate that at least one new BioBrick Part or Device of your own design and construction works as expected; characterize the operation of your new part/device.
  • Enter this information and other documentation on both the iGEM 2010 wiki and the Registry.
    • Part Number(s): BBa_K398206
    • iGEM Wiki Page Name: Team:TU_Delft/Parts/characterization

Requirements for a Gold Medal: (one OR more)

While waiting for our DNA to be synthesized we decided to spend the time on characterizing bricks from the distribution plate.

    • Part Number(s): [http://partsregistry.org/Part:BBa_J61100 BBa_J61100], [http://partsregistry.org/Part:BBa_J61101 BBa_J61101], [http://partsregistry.org/Part:BBa_J61107 BBa_J61101], [http://partsregistry.org/Part:BBa_J61117 BBa_J61101], [http://partsregistry.org/Part:BBa_J61127 BBa_J61101]
  • Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system. human practice research and wiki development.

We were happy to share the source code of our wiki with many iGEM Teams. Some tips and tricks have summarized on our Wiki Tips and Tricks page.

  • Develop and document a new technical standard that supports the: (check all that apply)
    • design of BioBrick Parts or Devices

For the design of our BioBricks we developed a protocol that guides you through getting the sequence from GenBank, optimizing it for E. coli expression, removing unwanted restriction sites and coupling it to the BioBrick prefix and suffix.

    • construction of BioBrick Parts or Devices

Since the standard 3-way ligation protocol gave us a lot of trouble, we came up with a new protocol for the construction of BioBricks. The protocol is basically a 2-way ligation, in which you leave the backbone of the recieving plasmid intact.

    • characterization of BioBrick Parts or Devices

For the characterization of the emulsifier and the alkane degradation biobricks, we developed our own assays.

    • analysis, modeling, and simulation of BioBrick Parts or Devices

Our team made a new bio-informatics tool for the prediction of protein interactions based on homology, called HIM.

    • Please provide the BBF RFC number for your standard:
  • Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.

Link to this information on your wiki. Page name: Team:TU_Delft/Human-practice Besides work in the lab, we spend a lot of time on education, publicity and ethics. This resulted in a new iGEM video game, workshops, interviews with professors and much media attention.

iGEM Prizes

  • Best Human Practice Advance

Explanation:

Team_Parts Part Number(s): Not decided which ones yet

iGEM Safety Page name: Team:TU_Delft/Safety/in-the-lab

Attribution and Contributions Page name: Team:TU_Delft/Collaboration/acknowledgements

Comments Page name: Team:TU_Delft/Tour

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