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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/15
From 2010.igem.org
(→12:Transformation) |
(→13:Electrophoresis) |
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<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Transformation_with_ECOS_competent_E-coli.28NIPPON_GENE.29/ Protocol 7 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Transformation_with_ECOS_competent_E-coli.28NIPPON_GENE.29/ Protocol 7 ] | ||
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===13:Electrophoresis=== | ===13:Electrophoresis=== | ||
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<Sample><br /> | <Sample><br /> | ||
| - | *1-2-3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2-3] |
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | *6-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6-7] |
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
| - | *1 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
| - | + | ||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | ||
| - | |||
<Result><br /> | <Result><br /> | ||
[[Image:Matsuura_2010-10-16_03hr_24min.JPG]] | [[Image:Matsuura_2010-10-16_03hr_24min.JPG]] | ||
| - | |||
===14:Electrophoresis=== | ===14:Electrophoresis=== | ||
Revision as of 12:43, 19 October 2010
Contents |
2010/10/15
1：DNA extraction
＜Member＞
Hitomi
＜Sample＞
＜Protocol＞
SeeProtocol 4
2:Electrophoresis
<Member>
Hitomi
<Sample>
<Protocol>
See Protocol 8
<Result>
3：DNA extraction from gels
＜Member＞
Mariko
＜Sample＞
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
4:PCR
<Member>
Hitomi
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 2
5:Electrophoresis
<Member>
nito
<Sample>
<Protocol>
See Protocol 8
<Result>
6:PCR
<Member>
mio
<Sample>
<Protocol>
See Protocol 2
7：DNA Digestion
＜Member＞
Hitomi
＜Sample, Materials＞
- vector
- 9-7
＜Protocol＞
See Protocol 9
8：DNA Ligation
＜Member＞
Hitomi
＜Sample＞
＜Protocol＞
See Protocol 3
9:PCR
<Member>
mio
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 2
10:PCR
<Member>
nito,mizuki
<Sample>
- 9
- vector
<Protocol>
See Protocol 2
11:Electrophoresis
<Member>
mio
<Sample>
<Protocol>
See Protocol 8
<Result>
12:Transformation
<Member>
nito
<Sample>
<Protocol>
See Protocol 7
13:Electrophoresis
<Member>
nito
<Sample>
<Protocol>
See Protocol 8
<Result>
14:Electrophoresis
<Member>
nito
<Sample>
- 9
- vector
<Protocol>
See Protocol 8
<Result>
