Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/04

From 2010.igem.org

(Difference between revisions)
(2010/09/01)
(2:DNA Ligation)
Line 19: Line 19:
<Sample><br />
<Sample><br />
-
*63  
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6-3]  
-
*67
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6-7]
-
*102
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2]
-
*27
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-7]
-
*23
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-3]
-
*12
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]
-
*45
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5]
(after digestion)
(after digestion)
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
-
 
===3:PCR===
===3:PCR===

Revision as of 11:55, 19 October 2010


Contents

2010/10/04

1:DNA extraction

<Member>
nito

<Sample>
Digestion products (10/3)

<Protocol>
See Protocol 4


2:DNA Ligation

<Member>
nito,Hitomi

<Sample>

(after digestion)

<Protocol>
See Protocol 10

3:PCR

<Member>
nito,Takahiro

<Sample>(temperature in annealing:℃)
Ligated 10/3,10/4

<Protocol>
See Protocol 1


4:PCR

<Member>
Mariko,Takahiro,nito

<Sample>(temperature in annealing:℃)

  • 7-8-10

<Protocol>
See Protocol 1