Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/03
From 2010.igem.org
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<Sample><br /> | <Sample><br /> | ||
- | *7-8-10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10] |
*JM109 | *JM109 | ||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Transformation_with_ECOS_competent_E-coli.28NIPPON_GENE.29/ Protocol 7 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Transformation_with_ECOS_competent_E-coli.28NIPPON_GENE.29/ Protocol 7 ] | ||
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===7:DNA Digestion=== | ===7:DNA Digestion=== |
Revision as of 11:54, 19 October 2010
![](https://static.igem.org/mediawiki/2010/9/9f/Re-main.png)
Contents |
2010/10/03
1:PCR
<Member>
hito
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 2
2:DNA extraction
<Member>
mio
<Sample>
PCR products
<Protocol>
See Protocol 4
3:Electrophoresis
<Member>
nito
<Sample>
All PCR products(10/3)
<Protocol>
See Protocol 8
4:DNA extraction
<Member>
mio,mizuki,nito
<Sample>
PCR products
Digestion products
<Protocol>
See Protocol 4
5:DNA Ligation
<Member>
mio,mizuki
<Sample>
(after digestion)
<Protocol>
See Protocol 10
6:Transformation
<Member>
nito
<Sample>
- 7-8-10
- JM109
<Protocol>
See Protocol 7
7:DNA Digestion
<Member>
nito
<Sample, Materials>
・PCR productions
- 1
- 2
- 3
- 4
- 6
- 10
・Digest enzyme
- Avr2
- Nhe1
<Protocol>
See Prptpcpl 9