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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/03
From 2010.igem.org
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＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *1-2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] |
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | *6A-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6A-7] |
| - | *7-8-10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10] |
(after digestion) | (after digestion) | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | ||
| - | |||
===6:Transformation=== | ===6:Transformation=== | ||
Revision as of 11:53, 19 October 2010
Contents |
2010/10/03
1:PCR
<Member>
hito
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 2
2：DNA extraction
＜Member＞
mio
＜Sample＞
PCR products
＜Protocol＞
See Protocol 4
3:Electrophoresis
<Member>
nito
<Sample>
All PCR products(10/3)
<Protocol>
See Protocol 8
<Result>
4：DNA extraction
＜Member＞
mio,mizuki,nito
＜Sample＞
PCR products
Digestion products
＜Protocol＞
See Protocol 4
5：DNA Ligation
＜Member＞
mio,mizuki
＜Sample＞
(after digestion)
＜Protocol＞
See Protocol 10
6:Transformation
<Member>
nito
<Sample>
- 7-8-10
- JM109
<Protocol>
See Protocol 7
7：DNA Digestion
＜Member＞
nito
＜Sample, Materials＞
・PCR productions
- 1
- 2
- 3
- 4
- 6
- 10
・Digest enzyme
- Avr2
- Nhe1
＜Protocol＞
See Prptpcpl 9
