Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/29

From 2010.igem.org

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(→1：DNA extraction)
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<Sample><br />
<Sample><br />
PCR products (9/27)
PCR products (9/27)
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*1  
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
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*2
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
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*3
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3]
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*4
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4]
<Protocol><br />
<Protocol><br />

Revision as of 11:46, 19 October 2010


Contents

2010/09/29

1：DNA extraction

＜Member＞
nito

＜Sample＞
PCR products

  • 1 (9/27)①
  • 1 (9/27)②
  • 2 (9/27)
  • 3 (9/27)
  • 4 (9/27)①
  • 4 (9/27)②
  • 5(9/27)
  • 8 (9/27)
  • 10(9/27)

Digestion products (9/27)

＜Protocol＞
See Protocol 4

2:Electrophoresis

<Member>
nito

<Sample>
PCR products (9/27)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-29 14hr 34min.JPG

3：DNA Ligation

＜Member＞
nito

＜Sample＞

  • 1,2
  • 4,5

(after digestion)

＜Protocol＞
See Protocol 3


4:PCR

<Member>
mio,mizuki

<Sample>(temperature in annealing:℃)

  • 5 (70)
  • 6 (63)
  • 7-8(70)
  • 9 (58,63,69,70,72)
  • 10 (58)
  • 1-2(72)

<Protocol>
See Protocol 1
But today we used Kod.

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