UTDallas/30 September 2010

From 2010.igem.org


Notebook

June
MTWTFSS
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7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
November
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30


September 30, 2010

  • Incubate the PyeaR+reporters in 0, 10uM, 100uM, and 1mM potassium nitrate solutions, then observed under the fluorescence microscope.
  • The intensity of the fluorescence increased with increasing molarity of nitrate solution.
  • Plan to do a larger scale titration test to get better data.
  • Miniprepped, digested, ran gel electrophoresis, and gel purified Pu.
  • Made LB carb. broth and more carb. plates.
9-30.JPG

Alkane Sensor

  • Had colonies from yesterday's transformation
  • Incubated transformants for miniprepping