UTDallas/23 September 2010

From 2010.igem.org


Notebook

June
MTWTFSS
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7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
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4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
November
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30


September 23, 2010

  • Prepared glycerol stock of the transformed ligated parts, in case they are correct.
  • Miniprepped the transformed ligated parts, and then digested it.
  • We also digested another part using all the enzymes to test the enzymes, because we have gotten poor digestions in the past.
  • We ran all the digestions on two gels. (pictures are attached)
  • The first gel is the digested parts. They are not correct, therefore are ligation did not work. We will try again tomorrow.
  • The second gel is the enzymes. The resulting gel was strange, especially the two middle results. This did not tell us much.
9-23.JPG
9-23(2).JPG

Alkane Sensor

  • Gel Purified PalkB