UTDallas/16 October 2010

From 2010.igem.org


Notebook

June
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7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
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2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
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6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
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4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
November
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8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30


October 16, 2010

  • Made chloramphenicol plates
  • Transformed the ligations from yesterday.
  • I also realized that the ligations will give us the wrong product. We took a random part (BBa_J04450) with the pSB1C3 backbone to digest to get just the backbone, but I did not digest it properly, so the ligations will give us our parts (PyeaR+GFP and PyeaR+RFP) behind LacI+RFP. Therefore I will be digesting and ligating again during the week.

Alkane Sensor

  • Transformed ligation parts from yesterday