UTDallas/15 September 2010
Notebook
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September 15, 2010
- the transformations did not work from yesterday, so we decided to redigest the parts to make certain that they were actually cut with both enzymes.
- We then ran some gels, both gels had the appropriate results so we gel purified and then ligated.
- We also tested the Pu+GFP+Pr+XylR broths for fluorescence again, and the negative control is still as fluorescent as the others. We will try again later.
- We incubated from glycerol stock most of the parts used today for miniprepping tomorrow.
The gels: