Team:Warsaw/Calendar-Stage2/21 September 2010
From 2010.igem.org
Purification of MinC expression vectors
Kuba
Six colonies were picked for inoculation of liquid cultures (three with B0032 and three with B0034). Cultures were grown for 6 h in 37 oC. Plasmids were purified with Plasmid Mini kit (A&A) biotechnology. The obtained sample, as well as the initial (unmutated) plasmid, were then digested with EcoRI and PstI for 1 h and run on agarose gel.
M - size marker; 32a,b,c - plasmids harboring MinC under weak RBS; 34a,b,c - plasmids harboring MinC under strong RBS
Samples 32a,b&c ans well as 34b&c appear to contain a correct construct. Smaller amounts of DNA with B0034-containing constructs may indicate a slight leaking of the construct (probably due to a read-through from the resistance gene).