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Team:Warsaw/Calendar-Stage1/16 August 2010
From 2010.igem.org
Expression of mCherryNLS from the distribution
1. Transformation with 2ul DNA from the distribution.
Cloning Fluorescent proteins behind expression vector J23100-AndersonRBS sample 3.1
1. EcoRI/Pst digest of obtained clones to check size of the constructs, samples:
GFPz3
CFP1
CFPz1
MOz1
mOrrange1
MC2
mCherry1
mCherryNLSz1
2. GEL
Cloning Fluorescent proteins behind J23100 (without RBS).
1. Ligation ofJ23100 with YFP, mCherry, mOrrange.
2. Transformation with ligation mixture
