Team:UPO-Sevilla/Notebook/10 22

From 2010.igem.org

October, 22nd

Assay Team

Chemotaxis assay: we sterilized flow-chambers and prepared an Adler's chamber; we also prepared capillaries with aspartate and TQ, and carried out the experiment in the same way that the time before, but now in flow-chambers.

We put 200ul of cellular suspension in Adler's chamber and we observed by microscopy every 15 minutes, from 0' to 60'. There were more bacteria in aspartate capillaries.

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