Dry Experiments Records
July
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M | T | W | T | F | S | S
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| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/1_July_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/2_July_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/3_July_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/4_July_2010&action=edit 4]
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[http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/5_July_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/6_July_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/7_July_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/8_July_2010&action=edit 8]
| [http://2010.igem.org/Team:Tsinghua/Notebook/9_July_2010 9]
| [http://2010.igem.org/Team:Tsinghua/Notebook/10_July_2010 10]
| [http://2010.igem.org/Team:Tsinghua/Notebook/11_July_2010 11]
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[http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/12_July_2010&action=edit 12]
| [http://2010.igem.org/Team:Tsinghua/Notebook/13_July_2010 13]
| [http://2010.igem.org/Team:Tsinghua/Notebook/14_July_2010 14]
| [http://2010.igem.org/Team:Tsinghua/Notebook/15_July_2010 15]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/16_July_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/17_July_2010&action=edit 17]
| [http://2010.igem.org/Team:Tsinghua/Notebook/18_July_2010 18]
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[http://2010.igem.org/Team:Tsinghua/Notebook/19_July_2010 19]
| [http://2010.igem.org/Team:Tsinghua/Notebook/20_July_2010 20]
| [http://2010.igem.org/Team:Tsinghua/Notebook/21_July_2010 21]
| [http://2010.igem.org/Team:Tsinghua/Notebook/22_July_2010 22]
| [http://2010.igem.org/Team:Tsinghua/Notebook/23_July_2010 23]
| [http://2010.igem.org/Team:Tsinghua/Notebook/24_July_2010 24]
| [http://2010.igem.org/Team:Tsinghua/Notebook/25_July_2010 25]
|
[http://2010.igem.org/Team:Tsinghua/Notebook/26_July_2010 26]
| [http://2010.igem.org/Team:Tsinghua/Notebook/27_July_2010 27]
| [http://2010.igem.org/Team:Tsinghua/Notebook/28_July_2010 28]
| [http://2010.igem.org/Team:Tsinghua/Notebook/29_July_2010 29]
| [http://2010.igem.org/Team:Tsinghua/Notebook/30_July_2010 30]
| [http://2010.igem.org/Team:Tsinghua/Notebook/31_July_2010 31]
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August
|
M | T | W | T | F | S | S
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| [http://2010.igem.org/Team:Tsinghua/Notebook/1_August_2010 1]
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[http://2010.igem.org/Team:Tsinghua/Notebook/2_August_2010 2]
| [http://2010.igem.org/Team:Tsinghua/Notebook/3_August_2010 3]
| [http://2010.igem.org/Team:Tsinghua/Notebook/4_August_2010 4]
| [http://2010.igem.org/Team:Tsinghua/Notebook/5_August_2010 5]
| [http://2010.igem.org/Team:Tsinghua/Notebook/6_August_2010 6]
| [http://2010.igem.org/Team:Tsinghua/Notebook/7_August_2010 7]
| [http://2010.igem.org/Team:Tsinghua/Notebook/8_August_2010 8]
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[http://2010.igem.org/Team:Tsinghua/Notebook/9_August_2010 9]
| [http://2010.igem.org/Team:Tsinghua/Notebook/10_August_2010 10]
| [http://2010.igem.org/Team:Tsinghua/Notebook/11_August_2010 11]
| [http://2010.igem.org/Team:Tsinghua/Notebook/12_August_2010 12]
| [http://2010.igem.org/Team:Tsinghua/Notebook/13_August_2010 13]
| [http://2010.igem.org/Team:Tsinghua/Notebook/14_August_2010 14]
| [http://2010.igem.org/Team:Tsinghua/Notebook/15_August_2010 15]
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[http://2010.igem.org/Team:Tsinghua/Notebook/16_August_2010 16]
| [http://2010.igem.org/Team:Tsinghua/Notebook/17_August_2010 17]
| [http://2010.igem.org/Team:Tsinghua/Notebook/18_August_2010 18]
| [http://2010.igem.org/Team:Tsinghua/Notebook/19_August_2010 19]
| [http://2010.igem.org/Team:Tsinghua/Notebook/20_August_2010 20]
| [http://2010.igem.org/Team:Tsinghua/Notebook/21_August_2010 21]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/22_August_2010&action=edit 22]
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[http://2010.igem.org/Team:Tsinghua/Notebook/23_August_2010 23]
| [http://2010.igem.org/Team:Tsinghua/Notebook/24_August_2010 24]
| [http://2010.igem.org/Team:Tsinghua/Notebook/25_August_2010 25]
| [http://2010.igem.org/Team:Tsinghua/Notebook/26_August_2010 26]
| [http://2010.igem.org/Team:Tsinghua/Notebook/27_August_2010 27]
| [http://2010.igem.org/Team:Tsinghua/Notebook/28_August_2010 28]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/29_August_2010&action=edit 29]
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[http://2010.igem.org/Team:Tsinghua/Notebook/30_August_2010 30]
| [http://2010.igem.org/Team:Tsinghua/Notebook/31_August_2010 31]
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September
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M | T | W | T | F | S | S
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| [http://2010.igem.org/Team:Tsinghua/Notebook/1_September_2010 1]
| [http://2010.igem.org/Team:Tsinghua/Notebook/2_September_2010 2]
| [http://2010.igem.org/Team:Tsinghua/Notebook/3_September_2010 3]
| [http://2010.igem.org/Team:Tsinghua/Notebook/4_September_2010 4]
| [http://2010.igem.org/Team:Tsinghua/Notebook/5_September_2010 5]
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[http://2010.igem.org/Team:Tsinghua/Notebook/6_September_2010 6]
| [http://2010.igem.org/Team:Tsinghua/Notebook/7_September_2010 7]
| [http://2010.igem.org/Team:Tsinghua/Notebook/8_September_2010 8]
| [http://2010.igem.org/Team:Tsinghua/Notebook/9_September_2010 9]
| [http://2010.igem.org/Team:Tsinghua/Notebook/10_September_2010 10]
| [http://2010.igem.org/Team:Tsinghua/Notebook/11_September_2010 11]
| [http://2010.igem.org/Team:Tsinghua/Notebook/12_September_2010 12]
|
[http://2010.igem.org/Team:Tsinghua/Notebook/13_September_2010 13]
| [http://2010.igem.org/Team:Tsinghua/Notebook/14_September_2010 14]
| [http://2010.igem.org/Team:Tsinghua/Notebook/15_September_2010 15]
| [http://2010.igem.org/Team:Tsinghua/Notebook/16_September_2010 16]
| [http://2010.igem.org/Team:Tsinghua/Notebook/17_September_2010 17]
| [http://2010.igem.org/Team:Tsinghua/Notebook/18_September_2010 18]
| [http://2010.igem.org/Team:Tsinghua/Notebook/19_September_2010 19]
|
[http://2010.igem.org/Team:Tsinghua/Notebook/20_September_2010 20]
| [http://2010.igem.org/Team:Tsinghua/Notebook/21_September_2010 21]
| [http://2010.igem.org/Team:Tsinghua/Notebook/22_September_2010 22]
| [http://2010.igem.org/Team:Tsinghua/Notebook/23_September_2010 23]
| [http://2010.igem.org/Team:Tsinghua/Notebook/24_September_2010 24]
| [http://2010.igem.org/Team:Tsinghua/Notebook/25_September_2010 25]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/26_September_2010&action=edit 26]
|
[http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/27_September_2010&action=edit 27]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/28_September_2010&action=edit 28]
| [http://2010.igem.org/Team:Tsinghua/Notebook/29_September_2010 29]
| [http://2010.igem.org/Team:Tsinghua/Notebook/30_September_2010 30]
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October
|
M | T | W | T | F | S | S
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| [http://2010.igem.org/Team:Tsinghua/Notebook/1_October_2010 1]
| [http://2010.igem.org/Team:Tsinghua/Notebook/2_October_2010 2]
| [http://2010.igem.org/Team:Tsinghua/Notebook/3_October_2010 3]
|
[http://2010.igem.org/Team:Tsinghua/Notebook/4_October_2010 4]
| [http://2010.igem.org/Team:Tsinghua/Notebook/5_October_2010 5]
| [http://2010.igem.org/Team:Tsinghua/Notebook/6_October_2010 6]
| [http://2010.igem.org/Team:Tsinghua/Notebook/7_October_2010 7]
| [http://2010.igem.org/Team:Tsinghua/Notebook/8_October_2010 8]
| [http://2010.igem.org/Team:Tsinghua/Notebook/9_October_2010 9]
| [http://2010.igem.org/Team:Tsinghua/Notebook/10_October_2010 10]
|
[http://2010.igem.org/Team:Tsinghua/Notebook/11_October_2010 11]
| [http://2010.igem.org/Team:Tsinghua/Notebook/12_October_2010 12]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/13_October_2010&action=edit 13]
| [http://2010.igem.org/Team:Tsinghua/Notebook/14_October_2010 14]
| [http://2010.igem.org/Team:Tsinghua/Notebook/15_October_2010 15]
| [http://2010.igem.org/Team:Tsinghua/Notebook/16_October_2010 16]
| [http://2010.igem.org/Team:Tsinghua/Notebook/17_October_2010 17]
|
[http://2010.igem.org/Team:Tsinghua/Notebook/18_October_2010 18]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/19_October_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/20_October_2010&action=edit 20]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/21_October_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/22_October_2010&action=edit 22]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/23_October_2010&action=edit 23]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/24_October_2010&action=edit 24]
|
[http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/25_October_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/26_October_2010&action=edit 26]
| [http://2010.igem.org/Team:Tsinghua/Notebook/27_October_2010 27]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/28_October_2010&action=edit 28]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/29_October_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/30_October_2010&action=edit 30]
| [http://2010.igem.org/wiki/index.php?title=Team:Tsinghua/Notebook/31_October_2010&action=edit 31]
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Our Experiments were carried out by the following nine groups which are somewhat independent from each other. Here come the Groups and Their Tasks.
Group 1A:Landing pad (LP) construction and Insertion with Tranditional Att Recomination method(Promoter Inside)
Group 1B:Landing pad (LP) construction and Insertion with Innovational Att Recomination method(Promoter Outside)
Group 1C:Landing pad (LP) construction and Insertion: Inducement and Recombination
Group 2A:Donor Plasmid Construction: Two Fragments Construction (Three Fragments Ligation)
Group 2B:Donor Plasmid Construction: Traditional Four Fragments Construction
Group 2C:Donor Plasmid Construction: Four Fragments Construction with Dra III
Group 2D:Donor Plasmid Construction: Four Fragments Construction with Novel Methods
Group 3:Helper Plasmid (HP) Insertion, Donor Plasmid Insertion, Recombination Induction & Removal of HP
Group 4:Screening Strategy