From 2010.igem.org

DNA order

Aljoscha signing the DNA order

After the relaxing jam session of yesterday, we all head back to the lab/office. Besides planning the trip to Paris workshop for this weekend, we also spend some time on setting up the first transformations. Also new possible sponsors are addressed. We are still on the hunt!

The ordering invoice for the DNA synthesis are signed and on their way to Mr Gene. Of course we added their logo to our webpages [http://www.igemtudelft.nl igemtudelft.nl] and [http://tiny.cc/igemtudelft Facebook] and [http://twitter.com/igemtudelft2010 Twitter]. And all team members have become fans on the network sites.

Lab work

Antibiotica stocks

Hugo, Ramon and Pieter made the antibiotic stock solutions. Though it is kinda hard to solubilize Chloramphenicol in water. Fortunately we have Hugo, the vortexman, on our team!

BioBrick stocks

We suspended some BioBricks from the distribution plate that we are planning to use in our project. Some of the BioBricks contain RFP. The red colour in our epps really looked nice! De BioBricks that we suspended were:

[http://partsregistry.org/Part:pSB1A3 pSB1A3]
[http://partsregistry.org/Part:pSB1K3 pSB1K3]
[http://partsregistry.org/Part:pSB1T3 pSB1T3]
[http://partsregistry.org/Part:pSB1C3 pSB1C3]
[http://partsregistry.org/Part:pSB3T5 pSB3T5]
[http://partsregistry.org/Part:pSB3C5 pSB3C5]
[http://partsregistry.org/Part:I13401 I13401]
[http://partsregistry.org/Part:B0015 B0015]
[http://partsregistry.org/Part:B0032 B0032]
[http://partsregistry.org/Part:B0034 B0034]
[http://partsregistry.org/Part:E0040 E0040]
[http://partsregistry.org/Part:E0240 E0240]
[http://partsregistry.org/Part:E0422 E0422]
[http://partsregistry.org/Part:J61100 J61100]
[http://partsregistry.org/Part:J61101 J61101]
[http://partsregistry.org/Part:J61107 J61107]
[http://partsregistry.org/Part:J61117 J61117]
[http://partsregistry.org/Part:J61127 J61127]
[http://partsregistry.org/Part:J23109 J23109]
[http://partsregistry.org/Part:R0010 R0010]
[http://partsregistry.org/Part:R0011 R0011]

The concentration of the suspended BioBricks is not sufficient for all our plans. To make more plasmid, we are going to transform the BioBricks, grow colonies and isolate the plasmid.

Thias is also preparing for PCR amplification of a few biobricks. Esengül explained the mysteries of the magnificant PCR machines.