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From 2010.igem.org

Johan

Colony PCR screen

bFGF-his and his-bFGF in pMA (vector with histag)

0,5 µl pol

0,5 µl dNTP

5 µl 10x buffer

1,5 µl pMA for primer

1,5 µl pMA rev primer

16 µl H2O

10 colonies from both plates

Gel

Results: All colonies correct! Expected as both insert and vector had been gel purified.