Team:Stockholm/26 September 2010



Colony PCR screen

bFGF-his and his-bFGF in pMA (vector with histag)

0,5 µl pol

0,5 µl dNTP

5 µl 10x buffer

1,5 µl pMA for primer

1,5 µl pMA rev primer

16 µl H2O

10 colonies from both plates


SU 26sepgels.png

SU 26sepgels 1.png

Results: All colonies correct! Expected as both insert and vector had been gel purified.

The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/