22 July 2010

• Gel with products of digestion run --> only one of the base vector samples and a fragment with the origin and a Kan resistance worked. The others probably did not wok since the concentration of DNA was too low.
• Colonies picked and suspension cultures started. For making a miniprep tomorrow morning.
• Gel cut, Gel purification made
• New idea: PCR run on the product of the miniprep.
• Ligation of Base Vector with Origin+Kan
• Asaia preculture for OD growth (different pH) measurements
• Gly medium with pH 2,3,4,5,6,7.
• Research
• Replating of Asaia (Replating III) to get the WT (one day!!)