Team:EPF Lausanne/Calendar/0722
From 2010.igem.org
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22 July 2010
- Gel with products of digestion run --> only one of the base vector samples and a fragment with the origin and a Kan resistance worked. The others probably did not wok since the concentration of DNA was too low.
- Colonies picked and suspension cultures started. For making a miniprep tomorrow morning.
- Gel cut, Gel purification made
- New idea: PCR run on the product of the miniprep.
- Ligation of Base Vector with Origin+Kan
- Asaia preculture for OD growth (different pH) measurements
- Gly medium with pH 2,3,4,5,6,7.
- Research
- Replating of Asaia (Replating III) to get the WT (one day!!)
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