Team:Calgary/4 September 2010


Saturday September 4, 2010

Chris's restriction digest of random CpxP that Emily miniprepped/ Results are inconclusive

Weekends are awesome!! 1 week to aGEM!


Today, I ran a restriction digest of a bunch of the CpxP promoters that Emily miniprepped and ran them on a 1.0% gel. The results can be seen on the right. There are no real conclusive results so they were redigested and the gel will be run tomorrow. I also did plasmid preparations of a bunch of overnight cultures of ibpAB and CpxP that were set up yesterday.


This evening I helped Chris with miniprpeps of various cultues from yesterday. I also set up a colony PCR of new malESSdel colonies as well as malE and malE31 colonies.