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Team:Brown/Notebook/August17
From 2010.igem.org
Tuesday, August 17, 2010
Transformations:
- bglII-CI-pGEM
- bglII-AraC-pGEM
- bglII-Mnt-pGEM
- RFC control (in BL21s as we are running out of XL1Bs
Plated w/ 2% xGal, 2ul IPTG for blue white selection on Amp+ LB plates.
Gel
- Lab Ladder
- Wessel lab ladder
- LovTwo PCR
Gel extracted lovtwo PCR product. Nanodrop reads 18.9ng/ul with an abnormally high 260/280. Could be impurities/salts from the gel.
Ligation of LovTwo into pGEM
18ul total volume
- 8.5ul ligase buffer 2x
- 1ul T4 ligase
- 0.5ul pGEM vector
- 8ul insert (Lovtwo)
