Team:UPO-Sevilla/Notebook/10 08

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       <h1>October, 8th</h1>
       <h1>October, 8th</h1>
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       <h2>Assay Team</h2>
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       <h2>Assembly Team</h2>
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         <p>Minipreps of 16+3 different inocula and digestion of them (2h, 37ºC). Samples were run in a 0.8% agarose gel. UPO16+3/3T5 was confirmed.</p>
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         <p>Minipreps of 16+3 different inocula and digestion of them (2h, 37ºC). Samples were run in a 0.8% agarose gel. UPO16+3/3T5 was confirmed.</p>
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<p>Minipreps of UPO4, 5 and 11/1C3 inocula and digestion of them (2h, 37ºC). Samples were run in a 0.8% agarose gel.  We have to repeat these digestions because there were few DNA quantity. Digestion again.</p>
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<p>Minipreps and digestion of UPO4, 5 and 11/1C3 inocula (2h, 37ºC). Samples were run in a 0.8% agarose gel.  We had to repeat these digestions because there were little DNA quantity. Digestion again.</p>
<p>Transformation of 13+3/3T5 ligations in DHTα.</p>
<p>Transformation of 13+3/3T5 ligations in DHTα.</p>
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<p>Digestion of UPO 1+2 and 7+3 (2h, 37ºC). Preparations were run in a 0.8% agarose gel and purified using GFX. Ligation of 1+2+7+3. </p>
+
<p>Digestion of UPO 1+2 and 7+3 (2h, 37ºC). Preparations were run in a 0.8% agarose gel and purified by using GFX. Ligation of 1+2+7+3. </p>
<p>12+2 primers pair were denatured and renatured. UPO 16+3 was digested (to 3A and SA) and checked by electrophoresis gel. Spots were isolated and purified using GFX.
<p>12+2 primers pair were denatured and renatured. UPO 16+3 was digested (to 3A and SA) and checked by electrophoresis gel. Spots were isolated and purified using GFX.
</p>
</p>

Revision as of 15:25, 27 October 2010

October, 8th

Assembly Team

Minipreps of 16+3 different inocula and digestion of them (2h, 37ºC). Samples were run in a 0.8% agarose gel. UPO16+3/3T5 was confirmed.

Minipreps and digestion of UPO4, 5 and 11/1C3 inocula (2h, 37ºC). Samples were run in a 0.8% agarose gel. We had to repeat these digestions because there were little DNA quantity. Digestion again.

Transformation of 13+3/3T5 ligations in DHTα.

Digestion of UPO 1+2 and 7+3 (2h, 37ºC). Preparations were run in a 0.8% agarose gel and purified by using GFX. Ligation of 1+2+7+3.

12+2 primers pair were denatured and renatured. UPO 16+3 was digested (to 3A and SA) and checked by electrophoresis gel. Spots were isolated and purified using GFX.

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