October, 7th

Assay Team

Inocula of 16+3/3T5 different colonies were set up.

Inocula of UPO4, 5 and 11/1C3 different colonies were set up.

Digestion of UPO 13+3 (2h, 37ºC) to clone them at pSB3T5 (final vector of 12+2+13+3 circuit). Digestion was run in a 0.8% agarose gel and the positive spot was isolated and purified using GFX. The purification was quantified by Nanodrop. Ligation of 13+3/3T5.