Team:UPO-Sevilla/Notebook/08 06

From 2010.igem.org

(Difference between revisions)
Line 19: Line 19:
       <h2>Production Team</h2>
       <h2>Production Team</h2>
-
       <p>Minipreps were prepared from inocula. We digested with EcoRI and PstI for 2 hours at 37ºC. We run an electrophoresis of the digestion products and we observed 1'5 kb fragments. We've got fecI-fecR. Colony PCR seems not to be a reliable proof.</p>
+
       <p>Minipreps were prepared from inocula. We digested with EcoRI and PstI for 2 hours at 37ºC. We ran an electrophoresis of the digestion products and we observed 1'5 kb fragments. We've got fecI-fecR. Colony PCR seems not to be a reliable proof.</p>

Revision as of 23:39, 26 October 2010

August, 6th

Production Team

Minipreps were prepared from inocula. We digested with EcoRI and PstI for 2 hours at 37ºC. We ran an electrophoresis of the digestion products and we observed 1'5 kb fragments. We've got fecI-fecR. Colony PCR seems not to be a reliable proof.

Assembly Team

We used 3A method to build: 1+2+7+2, 11+2+13+3, 12+2+13+3, 11+2+16+3 and 12+2+16+3. The necessary steps are: digestion with accurate digestion enzyme, ligation and transformation. Plates were incubated at 37º over night.

Minpreps using inocula we set up the day before. Analytic digestion confirmed the next biobriks: UPO6, UPO7, UPO2+6, UPO12+2 and UPO11+2.

Go to 5th of August Go to 9th of August
Return to Notebook

Footer