Preparation week - Week 5

Monday 26th July

We started transformation of the DNA we need from the iGEM registry plates today. Followed by inoculation, miniprep, diagnostic digestions and gel electrophoresis, in order to obtain enough correct DNA for further assembly (ligation).

Currently, we have the following parts (confirmed by gel electrophoresis):

- Inducible promoter lacI.

- Possible hypoxia promoter from UCL iGEM 2009 mNarK.

- Positive feedback loop promoter, LasR regulated.

- Terminator.

- Kanamycin backbone for assembly.

- Chloramphenicol backbone for assembly.

- Amp&Kana backbone as back-up plan for assembly.

- Amp&Chlor backbone as back-up plan for assembly.

The missing important parts we need for ligation are:

- RBS + coding region of LasR + terminator

- Red dye pathway (or any other colour)

- Native hypoxia promoter NarK.

Tuesday 27th July

Wednesday 28th July

Thursday 29th July

This Thursday meeting started as a one time event..and became like a bad habit..but in a good way. The portico seams to provide a very inspiring atmosphere for the iGEM UCL members to discuss their weekly progress and the tasks assigned to each one. For once more, a lot of ideas regarding the project were circulated.