Preparation week - Week 5

Monday 26th July

We started transformation of the DNA we need from the iGEM registry plates today. WE had 11 parts to transform, so Xiang and the team had quit a tough few days ahead of them.

Tuesday 27th July

The results of the transformation on the previous day were confirmedd today.

Wednesday 28th July

Thursday 29th July

This Thursday meeting started as a one time event..and became like a bad habit..but in a good way. The portico seams to provide a very inspiring atmosphere for the iGEM UCL members to discuss their weekly progress and the tasks assigned to each one. For once more, a lot of ideas regarding the project were circulated.

Friday 30th July

In terms of lab work, Currently, we have the following parts (confirmed by gel electrophoresis):

Inducible promoter lacI

Possible hypoxia promoter from UCL iGEM 2009 mNarK. Positive feedback loop promoter, LasR regulated. Terminator. Kanamycin backbone for assembly. Chloramphenicol backbone for assembly. Amp&Kana backbone as back-up plan for assembly. Amp&Chlor backbone as back-up plan for assembly.

The missing important parts we need for ligation

RBS + coding region of LasR + terminator Red dye pathway (or any other colour) Native hypoxia promoter NarK.

Saturday

Sunday