Team:UCL London/Our BioBricks
From 2010.igem.org
OUR BIOBRICKS
The following table is that of all the Biobricks and parts that we have used over the course of iGem 2010. Also, it's worth mentioning that we are the very first iGEM team to ever have 1.2 kg of biomass!!
NEW PARTS SUBMITTED & CHARACTERISED
Part Name | Part Type | Description | Designer | Length(bp) |
---|---|---|---|---|
BBa_K301000 | Regulatory | Tac promoter, hybrid derived from trp and lac promoters | Xiang Chen | 73 |
BBa_K301001 | Device | pTAC with RFP reporter | Xiang Chen | 942 |
BBa_K301003 | Regulatory | T7 promoter | Xiang Chen | 23 |
BBa_K301005 | Coding | T7 RNA Polymerase | Xiang Chen | 1000 |
BBa_K301006 | Device | Low Oxygen Level Self-induction Genetic Circuit. NarK promoter | Xiang Chen | 3082 |
BBa_K301007 | Device | Low Oxygen Level Self-induction Genetic Circuit. mNarK promoter | Xiang Chen | 3032 |
BBa_K301008 | Device | Self-induction Genetic Circuit with an Inducible Promoter | Xiang Chen | 3016 |
BBa_K301010 | Composite | Self-induction Genetic Circuit | Xiang Chen | 2937 |
Check out our parts page to see the parts submitted in more detail UCL_London 2010 iGEM Team Parts.
EXISTING PARTS/DEVICES CHARACTERISED
Part Code | Part Name | Location | Backbone | Length(bp) |
---|---|---|---|---|
BBa_K239011 | Stress Light GFP Anaerobic Metabolism Detector 2 | 972 | ||
BBa_K239009 | GFP test devise for Spy promoter | 1045 | ||
BBa_K239015 | GFP test devise for DegP promoter | 1116 |
OTHER PARTS/DEVICES TRIED/USED
Part Code | Part Name | Location | Backbone | Length(bp) |
---|---|---|---|---|
BBa_R0010 | PROMOTER (lacI Regulated) | Plate1_1D | pSB1A2 | 200 |
BBa_K239005 | Nark | Eurogentec | pUC57 | 139 |
BBa_K239006 | mNark | Eurogentec | pUC57 | 89 |
BBa_R0079 | Promoter (LasR & PAI regulated) | Plate1_19P | pSB1A2 | 936 |
BBa_K274100 | VRDP | Plate3_6H | pSB1A2 | 3385 |
BBa_B0015 | Double Terminator | Plate1_23L | pSB1AK3 | 129 |
pSB1K3 | Kanamycin Resistance Backbone | Plate1_5A | pSB1K3+J04450 insert | 2206 |
pSB1T3 | Tetracycline Resistance Backbone | Plate 1_7A | pSB1T3+J04450 insert | 2463 |
pSB1C3 | Chloramphenicol Resistance Backbone | Plate 1_3A | pSB1C3+J04450 insert | 2072 |
BBa_R0011 | Promoter (lacI regulated, lambda pL hybrid) | Plate1_6G | pSB1A2 | 55 |
BBa_B0034 | RBS | Plate1_2M | pSB1A2 | 12 |
BBa_C0079 | lasR activator from P. aeruginosa PAO1(+LVA) | Plate1_14J | pSB2K3 | 756 |
pSB1AK3 | Amp+Kana | Plate1_11A | +J04450 insert | 3189 |
pSB1AT3 | Amp+Tetra | Plate1_13A | +J04450 insert | 3446 |
pSB1AC3 | Amp+Chlora | Plate1_9A | +J04450 insert | 3055 |
BBa_K274200 | CrtEBIY with rbs (Orange) | Plate3_6L | pSB1A2 | 4555 |
BBa_K274002 | Purple | Plate3_12B | pSB1T3 | 7345 |
BBa_K274003 | Dark Green | Plate3_20H | pSB1K3 | 6032 |
Plate3_20D | pSB1T3 | 6032 | ||
BBa_K274004 | Light Green | Plate3_20J | pSB1K3 | 6200 |
Plate3_22F | pSBT3 | 6200 | ||
BBa_I13507 | RBS+RFP+Terminator | Pate1_22O | pSB1A2 | 861 |