Team:UCL London/Glycerol Stock
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- | =='''Glycerol Stock | + | =='''Glycerol Stock'''== |
'''Materials''' | '''Materials''' |
Revision as of 14:01, 30 September 2010
Glycerol Stock
Materials
Glycerol
Transformed E.coli cells.
Method
1. Add 80µL glycerol into each labelled eppendorf.
2. Add 300µL E.coli suspension into the glycerol.
3. Vortex & store at -20°C.
4. Rest of the E.coli suspension are stored in the fridge.