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	'''Materials'''		'''Materials'''

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Revision as of 09:48, 17 August 2010

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2010/8/11 Wednesday (Naoto)

Experiment:Grow up a culture of A.xylinus

Member
easily and naoto

Materials

• A.xylinum JCM strain 7644
• media for A.xylinus(Open Wet Ware recommended)
• media for A.xylinus(JCM recommended) 100ml

glucoce 10g
yeast extract 1g
CaCO3 3g
DW 100ml
(add agar 1.5g for plates)

• ｒecovery solution for A.xylinus 100ml

peptone 0.5g
yeast extract 0.3g
DW 100ml

Procedure

1. prepared media (plates of Open Wet Ware recommended media were made yesterday)
2. autoclaved to sterilize media(121°C　20minute)
3. added ｒecovery solution to an ampule which had A.xylinus
4. streaked A.xylinus onto plates or in media
5. incubated at 26°C

Experiment:Grow up a culture of E.coli

Member
NEX,Bambi75 and watachin

Materials
LB plates

Procedure

1. streaked E.coli onto plates
2. incubated at 37°C